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Prepublished online as a Blood First Edition Paper on September 12, 2002; DOI 10.1182/blood-2002-02-0569. This Article Full Text (PDF) All Versions of this Article: 2002-02-0569v1 101/3/1155    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sasmono, R T. Articles by Hume, D. A Search for Related Content PubMed PubMed Citation Articles by Sasmono, R T. Articles by Hume, D. A Social Bookmarking                   What's this? Submitted February 21, 2002 Accepted September 4, 2002 A macrophage colony-stimulating factor receptor (CSF-1R)-green fluorescent protein transgene is expressed throughout the mononuclear phagocyte system of the mouse R Tedjo Sasmono, Delvac Oceandy, Jeffrey W Pollard, Wei Tong, Paul Pavli, Brandon J Wainwright, Michael C Ostrowski, S Roy Himes, and David A Hume* Institute for Molecular Bioscience, University of Queensland, Brisbane, QLD, Australia; ARC Special Research Centre for Functional and Applied Genomics, University of Queensland, Brisbane, QLD, Australia Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA Canberra Hospital, Woden, ACT, Australia Department of Molecular Genetics, Ohio State University, Columbus, OH, USA * Corresponding author; email: d.hume{at}imb.uq.edu.au. The c-fms gene encodes the receptor for macrophage colony-stimulating factor (CSF-1). The gene is expressed selectively in the macrophage and trophoblast cell lineages. Previous studies have indicated that sequences in intron 2 control transcript elongation in tissue-specific and regulated expression of c-fms. In humans, an alternative promoter was implicated in expression of the gene in trophoblasts. We show that in mice, c-fms transcripts in trophoblasts initiate from multiple points within the 2kb region flanking the first coding exon. A reporter gene construct containing 3.5kb of 5' flanking sequence and the downstream intron 2 directed expression of enhanced green fluorescent protein (EGFP) to both trophoblasts and macrophages. EGFP was detected in trophoblasts from the earliest stage of implantation examined at embryonic day 7.5. During embryonic development, EGFP highlighted the large numbers of c-fms positive macrophages, including those that originate from the yolk sac. In adult mice, EGFP location was consistent with known F4/80-positive macrophage populations, including Langerhans cells of the skin and permitted convenient sorting of isolated tissue macrophages from disaggregated tissue. Expression of EGFP in transgenic mice was dependent upon intron 2 as no lines with detectable EGFP expression were obtained where either all of intron 2, or a conserved enhancer element FIRE (the Fms Intronic Regulatory Element) was removed. We have therefore defined the elements required to generate myeloid and trophoblast-specific transgenes as well as a model system for the study of mononuclear phagocyte development and function. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. Menke, W. A. Rabacal, K. T. Byrne, Y. Iwata, M. M. Schwartz, E. R. Stanley, A. Schwarting, and V. R. Kelley Circulating CSF-1 Promotes Monocyte and Macrophage Phenotypes that Enhance Lupus Nephritis J. 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