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Prepublished online as a Blood First Edition Paper on July 12, 2002; DOI 10.1182/blood-2002-02-0657. This Article Full Text (PDF) All Versions of this Article: 2002-02-0657v1 100/10/3698    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Voehringer, D. Articles by Pircher, H. Search for Related Content PubMed PubMed Citation Articles by Voehringer, D. Articles by Pircher, H. Social Bookmarking                   What's this? Submitted February 28, 2002 Accepted July 2, 2002 Lack of proliferative capacity of human effector and memory T cells expressing killer cell lectin-like receptor G1 (KLRG1) David Voehringer, Marie Koschella, and Hanspeter Pircher* Immunology, Institute for Medical Microbiology and Hygiene, University of Freiburg, Freiburg, Germany * Corresponding author; email: pircher{at}UKL.uni-freiburg.de. Adaptive immunity necessitates proliferation of lymphocytes. In the mouse, we have previously shown that antigen-experienced T cells that have lost their proliferative potential express the killer cell lectin-like receptor G1 (KLRG1). By using a newly generated monoclonal antibody specific for human KLRG1, we now demonstrate that expression of KLRG1 also identifies T cells in humans which are capable of secreting cytokines but fail to proliferate after stimulation. Furthermore, our data show that proliferative incapacity of CD8 T cells correlates better with KLRG1 expression than with absence of the CD28 marker. In peripheral blood lymphocytes (PBL) from healthy adult donors, KLRG1 was expressed on 44±14 % of CD8 and 18±10 % of CD4 T cells. KLRG1 expression was restricted to antigen-experienced T cells and here, KLRG1+ cells were preferentially found in the CCR7- effector T cell pool. Beside T cells, a significant portion (~ 50%) of human NK cells expressed KLRG1. Interestingly, these KLRG1+ NK cells were found exclusively in the CD56dim NK cell subset. Thus, expression of KLRG1 identifies a subset of NK cells and antigen-experienced T cells in humans which lack proliferative capacity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J.-S. Wang and C.-K. 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