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Prepublished online as a Blood First Edition Paper on September 19, 2002; DOI 10.1182/blood-2002-03-0711. This Article Full Text (PDF) All Versions of this Article: 2002-03-0711v1 101/3/869    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kuci, S. Articles by Handgretinger, R. Search for Related Content PubMed PubMed Citation Articles by Kuci, S. Articles by Handgretinger, R. Social Bookmarking                   What's this? Submitted March 7, 2002 Accepted September 5, 2002 Identification of a novel class of human adherent CD34- stem cells that give rise to SCID-repopulating cells Selim Kuci*, Johannes T Wessels, Hans-Joerg Buehring, Karin Schilbach, Michael Schumm, Gabriele Seitz, Juergen Loeffler, Peter Bader, Paul G Schlegel, Dietrich Niethammer, and Rupert Handgretinger Division of Hematology/Oncology, University Children's Hospital, Tuebingen, Germany Division of Hematology and Oncology, University Medical Clinic, Tuebingen, Germany Department of Hematology and Oncology, University Children's Hospital, Wuerzburg, Germany Division of Stem Cell Transplantation, St. Jude Children's Research Hospital, Memphis, TN, USA * Corresponding author; email: smkuci{at}med.uni-tuebingen.de. Here we describe the in vitro generation of a novel adherent cell fraction derived from highly enriched, mobilized CD133+ peripheral blood cells after their culture with Flt3/Flk2 ligand and interleukin-6 for 3 to 5 weeks. These cells lack markers of hematopoietic stem cells, endothelial cells, mesenchymal cells, dendritic cells, and stromal fibroblasts. However, all adherent cells expressed the extracellular matrix molecule VE-Cadherin and the adhesion molecules CD54 and CD44. They were also positive for CD164 and CD172a (signal regulatory protein-:SIRP-), and for a stem cell antigen defined by the recently described antibody W7C5. Adherent cells can either spontaneously or upon stimulation with stem cell factor give rise to a transplantable, nonadherent CD133+CD34- stem-cell subset. These cells do not generate in vitro hematopoietic colonies. However, their transplantation into immunodeficient NOD/SCID mice induced a substantially higher long-term multilineage engraftment compared to that of freshly isolated CD34+ cells, suggesting that these cells are highly enriched in SCID-repopulating cells (SRCs). In addition to cells of the myeloid lineage, nonadherent CD34- cells were also able to give rise to human cells with B, T and NK cell- phenotype. Hence, these cells possess a distinct in vivo differentiation potential compared to that of CD34+ stem cells, and may therefore provide an alternative to CD34+ progenitor cells for transplantation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. V. Cox, P. Diamanti, R. S. Evely, P. R. Kearns, and A. Blair Expression of CD133 on leukemia-initiating cells in childhood ALL Blood, April 2, 2009; 113(14): 3287 - 3296. [Abstract] [Full Text] [PDF] S. Voo, J. Eggermann, M. Dunaeva, C. Ramakers-van Oosterhoud, and J. Waltenberger Enhanced functional response of CD133+ circulating progenitor cells in patients early after acute myocardial infarction Eur. Heart J., January 2, 2008; 29(2): 241 - 250. [Abstract] [Full Text] [PDF] G. De Visscher, I. Vranken, A. 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