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Prepublished online as a Blood First Edition Paper on July 5, 2002; DOI 10.1182/blood-2002-03-0712. This Article Full Text (PDF) All Versions of this Article: 2002-03-0712v1 100/10/3797    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Goel, M. S Articles by Diamond, S. L Search for Related Content PubMed PubMed Citation Articles by Goel, M. S Articles by Diamond, S. L Social Bookmarking                   What's this? Submitted March 6, 2002 Accepted June 26, 2002 Adhesion of normal erythrocytes at depressed venous shear rates to activated neutrophils, activated platelets, and fibrin polymerized from plasma Mukul S Goel and Scott L Diamond* Department of Chemical Engineering, University of Pennsylvania, Philadelphia, PA, USA * Corresponding author; email: sls{at}seas.upenn.edu. Deep vein thrombosis (DVT) is a low flow pathology often prevented by vascular compression to increase blood movement. We report new heterotypic adhesive interactions of normal erythrocytes operative at low wall shear rates (w) below 100 s-1. Adhesion at w = 50 s-1 of washed RBC to fibrinogen-adherent platelets was 4-fold less (p < 0.005) than to collagen-adherent platelets (279 ± 105 RBC/mm2). This GPVI-triggered adhesion was antagonized (> 80 % reduction) by soluble fibrinogen (3 mg/ml) and EDTA. RBC-platelet adhesion was reduced in half by antibodies against CD36 and GPIb, but not by antibodies against GPIIb/IIIa, vWF, TSP, P-selectin, ß1, v, or CD47. Adhesion of washed RBC to fibrinogen-adherent neutrophils was increased 6-fold in the presence of 20 mM fMLP to a level of 67 RBC per 100 neutrophils after 5 min at 50 s-1. RBC-neutrophil adhesion was diminished by anti-CD11b (76%), anti-RBC LW (ICAM4; 40%), or by EDTA (>80%), but not by soluble fibrinogen or antibodies against CD11a, CD11c, CD36, TSP, ß1, v, or CD47. RBC adhesion to activated platelets and activated neutrophils was prevented by wall shear stress above 1 dyne/cm2 (at 100 s-1). While washed RBC did not adhere to fibrin formed from purified fibrinogen, adhesion was marked when pure fibrin was pre-coated with TSP or when RBC were perfused over fibrin formed from recalcified plasma. Endothelial activation and unusually low flow may be a setting prone to receptor-mediated red blood cell adhesion to adherent neutrophils (or platelets/fibrin), all of which may contribute to DVT. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Sezer, A. Cimen, E. Aslanger, A. Elitok, B. Umman, Z. Bugra, E. Yormaz, C. Turkmen, I.s. Adalet, Y. Nisanci, et al. Effect of intracoronary streptokinase administered immediately after primary percutaneous coronary intervention on long-term left ventricular infarct size, volumes, and function. J. Am. Coll. Cardiol., September 15, 2009; 54(12): 1065 - 1071. [Abstract] [Full Text] [PDF] H. Oh and S. L. Diamond Ethanol Enhances Neutrophil Membrane Tether Growth and Slows Rolling on P-Selectin but Reduces Capture from Flow and Firm Arrest on IL-1-Treated Endothelium J. Immunol., August 15, 2008; 181(4): 2472 - 2482. [Abstract] [Full Text] [PDF] S. P. Downie, S. 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