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Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-03-0718. This Article Full Text (PDF) All Versions of this Article: 2002-03-0718v1 101/2/711    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cooling, L. L W Articles by Koerner, T. A W Search for Related Content PubMed PubMed Citation Articles by Cooling, L. L W Articles by Koerner, T. A W Social Bookmarking                   What's this? Submitted March 6, 2002 Accepted August 12, 2002 Glycosphingolipid expression in acute nonlymphocytic leukemia: common expression of shiga toxin and parvovirus B19 receptors on early myeloblasts Laura L W Cooling*, De Sheng Zhang, Stanley J Naides, and Theodore A W Koerner Department of Pathology, University of Michigan, Ann Arbor, MI, USA; Department of Pathology, University of Iowa, Iowa City, IA, USA Department of Pathology, University of Iowa, Iowa City, IA, USA Departments of Medicine, Microbiology, Immunology and Pharmacology, Milton S. Hershey Medical Center of Pennylvania State University, Hershey, PA, USA * Corresponding author; email: lcooling{at}med.umich.edu. Glycosphingolipids (GSL) are complex macromolecules on cell membranes, which have been shown to play a role in neutrophil differentiation, activation, phagocytosis and adhesion to both microorganisms and vascular endothelium. Because GSLs are often cryptic antigens on cell membranes, little is known regarding GSL expression in early myelopoiesis. To study the latter, myeloblasts were collected from patients with acute nonlymphocytic leukemia (ANLL) who required therapeutic leukocytopheresis for hyperleukocytosis. The neutral GSLs were isolated and identified by high performance thin layer chromatography (HPTLC), HPTLC-immunostaining, gas chromatography, nuclear magnetic resonance and fast atom bombardment-mass spectrometry. Like mature peripheral blood neutrophils, myeloblasts expressed glucosylceramide, lactosylceramide and the neolacto-family GSLs, lactotriaosylceramide and neolactotetraosylceramide. Unlike neutrophils and chronic myeloid leukemia, most ANLL samples also expressed the globo-series GSLs, globotriaosylceramide and globotetraosylceramide. Globo-GSL expression was strongly associated with a myeloblastic (ANLL M0-M2) and monoblastic phenotype (M5). A weak association was also noted with expression of either lymphoid (P<0.10) or early hematopoietic markers (TdT, CD34; P<0.10). Globo-positive ANLL samples bound both shiga toxin and parvovirus B19 on HPTLC-immunostaining. Based on these findings, we propose that neolacto- and globo-GSLs are expressed during early myeloid differentiation. Globotriaosylceramide expression on myeloblasts, and possibly myeloid stem cells, may have important implications for the use of shiga toxin as an ex vivo purging agent in autologous stem cell transplantation. Expression of globotetraosylceramide, the parvovirus B19 receptor, on myeloblasts may also explain the association between B19 infection, aplastic anemia and chronic neutropenia of childhood. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Shu, H. S. Murphy, L. Cooling, and J. A. Shayman An In Vitro Model of Fabry Disease J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2636 - 2645. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020