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Prepublished online as a Blood First Edition Paper on August 22, 2002; DOI 10.1182/blood-2002-03-0788. This Article Full Text (PDF) All Versions of this Article: 2002-03-0788v1 100/13/4420    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Okuno, Y. Articles by Tenen, D. G Search for Related Content PubMed PubMed Citation Articles by Okuno, Y. Articles by Tenen, D. G Social Bookmarking                   What's this? Submitted March 13, 2002 Accepted July 17, 2002 Distal elements are critical for human CD34 expression in vivo Yutaka Okuno, Claudia S Huettner, Hanna S Radomska, Victoria Petkova, Hiromi Iwasaki, Koichi Akashi, and Daniel G Tenen* Hematology/Oncology Division, Harvard Institutes of Medicine, Boston, MA, USA Department of Cancer Immunology and AIDS, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA, USA * Corresponding author; email: dtenen{at}caregroup.harvard.edu. The elements regulating gene expression in hematopoietic stem cells are still poorly understood. We previously reported that a 141 kb human CD34 transgene confers properly regulated human CD34 expression in transgenic mice. A construct with only the human CD34 promoter and 3' enhancer region is not sufficient, suggesting that critical distal elements are necessary for expression of the human CD34 gene. To further localize such elements, we analyzed deletion constructs of the human CD34 gene and evaluated their function in transgenic mice. Constructs harboring as little as 18 kb of 5' and 26 kb of 3' human CD34 flanking sequence conferred human expression in tissues of transgenic mice with a pattern similar to that of the 141 kb human transgene. In contrast, a construct harboring 10 kb of 5' and 17 kb of 3' human CD34 flanking sequence gave no expression. These data demonstrate that regions 10 to 18 kb upstream and/or 17 to 26 kb downstream of the human CD34 gene contain critical elements for human CD34 expression in vivo. Further functional analysis of these regions in transgenic mice will be crucial for understanding CD34 gene expression in hematopoietic stem and progenitor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. A. P. Goncalves, E. C. Lira, A. M. Baviera, P. Cao, N. M. Zanon, Z. 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