SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on September 19, 2002; DOI 10.1182/blood-2002-03-0796. This Article Full Text (PDF) All Versions of this Article: 2002-03-0796v1 101/3/877    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gu, Y.-C. Articles by Ekblom, M. Search for Related Content PubMed PubMed Citation Articles by Gu, Y.-C. Articles by Ekblom, M. Social Bookmarking                   What's this? Submitted March 13, 2002 Accepted September 5, 2002 Laminin isoform-specific promotion of adhesion and migration of human bone marrow progenitor cells Yu-Chen Gu, Jarkko Kortesmaa, Karl Tryggvason, Jenny Persson, Peter Ekblom, Sten-Eirik Jacobsen, and Marja Ekblom* Department of Cell and Molecular Biology, Lund University, Lund, Sweden Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, Sweden Department of Laboratory Medicine, Lund University, Lund, Sweden; Stem Cell Laboratory, Lund University, Lund, Sweden Department of Laboratory Medicine, Lund University, Lund, Sweden * Corresponding author; email: marja.ekblom{at}medkem.lu.se. Laminins are ß heterotrimeric extracellular proteins, which regulate cellular functions by adhesion to integrin and non-integrin receptors. Laminins containing 4 and 5 chains are expressed in bone marrow but their interactions with hematopoietic progenitors are unknown. We studied human bone marrow cell adhesion to laminin-10/11 (5ß11/5ß21), laminin-8 (4ß11), laminin-1 (1ß11 and fibronectin. 35-40% of CD34+ and CD34+CD38- stem and progenitor cells adhered to laminin-10/11 and 45-50% adhered to fibronectin, whereas they adhered less to laminin-8 and to laminin-1. Adhesion of CD34+CD38- cells to laminin-10/11 was maximal without integrin activation, whereas adhesion to other proteins was dependent on protein kinase C activation by TPA. FACS analysis showed expression of integrin 6 chain on the majority of CD 34+ and CD34+CD38- cells. Integrin 6 and ß1 chains were involved in binding of both cell fractions to laminin-10/11 and laminin-8. Laminin-10/11 was highly adhesive to lineage committed myelomonocytic and erythroid progenitor cells, and most lymphoid and myeloid cell lines studied, whereas laminin-8 was less adhesive. In functional assays, both laminin-8 and laminin-10/11 facilitated SDF-1 stimulated transmigration of CD34+ cells, by an integrin 6 receptor mediated mechanism. In conclusion, we demonstrate laminin isoform specific adhesive interactions with human bone marrow stem, progenitor and more differentiated cells. The cell-adhesive laminins affected migration of hematopoietic progenitors, suggesting a physiological role for laminins during hematopoiesis. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: T. D. Schreiber, C. Steinl, M. Essl, H. Abele, K. Geiger, C. A. Muller, W. K. Aicher, and G. Klein The integrin {alpha}9{beta}1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation Haematologica, November 1, 2009; 94(11): 1493 - 1501. [Abstract] [Full Text] [PDF] J. Grassinger, D. N. Haylock, M. J. Storan, G. O. Haines, B. Williams, G. A. Whitty, A. R. Vinson, C. L. Be, S. Li, E. S. Sorensen, et al. Thrombin-cleaved osteopontin regulates hemopoietic stem and progenitor cell functions through interactions with {alpha}9{beta}1 and {alpha}4{beta}1 integrins Blood, July 2, 2009; 114(1): 49 - 59. [Abstract] [Full Text] [PDF] M. O. Ports, R. B. Nagle, G. D. Pond, and A. E. Cress Extracellular Engagement of {alpha}6 Integrin Inhibited Urokinase-Type Plasminogen Activator-Mediated Cleavage and Delayed Human Prostate Bone Metastasis Cancer Res., June 15, 2009; 69(12): 5007 - 5014. [Abstract] [Full Text] [PDF] T. M. Tancred, A. R. Belch, T. Reiman, L. M. Pilarski, and J. Kirshner Altered Expression of Fibronectin and Collagens I and IV in Multiple Myeloma and Monoclonal Gammopathy of Undetermined Significance J. Histochem. Cytochem., March 1, 2009; 57(3): 239 - 247. [Abstract] [Full Text] [PDF] G. Gorfu, I. Virtanen, M. Hukkanen, V.-P. Lehto, P. Rousselle, E. Kenne, L. Lindbom, R. Kramer, K. Tryggvason, and M. Patarroyo Laminin isoforms of lymph nodes and predominant role of {alpha}5-laminin(s) in adhesion and migration of blood lymphocytes J. Leukoc. Biol., September 1, 2008; 84(3): 701 - 712. [Abstract] [Full Text] [PDF] H. Qian, E. Georges-Labouesse, A. Nystrom, A. Domogatskaya, K. Tryggvason, S. E. W. Jacobsen, and M. Ekblom Distinct roles of integrins {alpha}6 and {alpha}4 in homing of fetal liver hematopoietic stem and progenitor cells Blood, October 1, 2007; 110(7): 2399 - 2407. [Abstract] [Full Text] [PDF] C. Selleri, P. Ragno, P. Ricci, V. Visconte, N. Scarpato, M. V. Carriero, B. Rotoli, G. Rossi, and N. Montuori The metastasis-associated 67-kDa laminin receptor is involved in G-CSF-induced hematopoietic stem cell mobilization Blood, October 1, 2006; 108(7): 2476 - 2484. [Abstract] [Full Text] [PDF] H. Bonig, K.-H. Chang, B. Nakamoto, and T. Papayannopoulou The p67 laminin receptor identifies human erythroid progenitor and precursor cells and is functionally important for their bone marrow lodgment Blood, August 15, 2006; 108(4): 1230 - 1233. [Abstract] [Full Text] [PDF] H. Qian, K. Tryggvason, S. E. Jacobsen, and M. Ekblom Contribution of {alpha}6 integrins to hematopoietic stem and progenitor cell homing to bone marrow and collaboration with {alpha}4 integrins Blood, May 1, 2006; 107(9): 3503 - 3510. [Abstract] [Full Text] [PDF] H. Qian, S. E. W. Jacobsen, and M. Ekblom Integrin alpha 6 Is Essential for Fetal Hematopoietic Progenitor, but Not Fetal Stem Cell Homing to Bone Marrow. Blood (ASH Annual Meeting Abstracts), November 16, 2005; 106(11): 1387 - 1387. [Abstract] B. L. Eckhardt, B. S. Parker, R. K. van Laar, C. M. Restall, A. L. Natoli, M. D. Tavaria, K. L. Stanley, E. K. Sloan, J. M. Moseley, and R. L. Anderson Genomic Analysis of a Spontaneous Model of Breast Cancer Metastasis to Bone Reveals a Role for the Extracellular Matrix Mol. Cancer Res., January 1, 2005; 3(1): 1 - 13. [Abstract] [Full Text] [PDF] H. Qian, S. E. W. Jacobsen, and M. Ekblom Involvement of the Integrin Alpha 6 Receptor in the Homing and Engraftment of Hematopoietic Stem and Progenitor Cells to Bone Marrow. Blood (ASH Annual Meeting Abstracts), November 16, 2004; 104(11): 1293 - 1293. [Abstract] Z. Wondimu, T. Geberhiwot, S. Ingerpuu, E. Juronen, X. Xie, L. Lindbom, M. Doi, J. Kortesmaa, J. Thyboll, K. Tryggvason, et al. An endothelial laminin isoform, laminin 8 ({alpha}4{beta}1{gamma}1), is secreted by blood neutrophils, promotes neutrophil migration and extravasation, and protects neutrophils from apoptosis Blood, September 15, 2004; 104(6): 1859 - 1866. [Abstract] [Full Text] [PDF] M. Maatta, A. Liakka, S. Salo, K. Tasanen, L. Bruckner-Tuderman, and H. Autio-Harmainen Differential Expression of Basement Membrane Components in Lymphatic Tissues J. Histochem. Cytochem., August 1, 2004; 52(8): 1073 - 1081. [Abstract] [Full Text] [PDF] M. Ferletta, Y. Kikkawa, H. Yu, J. F. Talts, M. Durbeej, A. Sonnenberg, R. Timpl, K. P. Campbell, P. Ekblom, and E. Genersch Opposing Roles of Integrin {alpha}6A{beta}1 and Dystroglycan in Laminin-mediated Extracellular Signal-regulated Kinase Activation Mol. Biol. Cell, May 1, 2003; 14(5): 2088 - 2103. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020