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Prepublished online as a Blood First Edition Paper on August 29, 2002; DOI 10.1182/blood-2002-03-0896. This Article Full Text (PDF) All Versions of this Article: 2002-03-0896v1 101/2/576    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Rossi, M. I. D Articles by Kincade, P. W Search for Related Content PubMed PubMed Citation Articles by Rossi, M. I. D Articles by Kincade, P. W Social Bookmarking                   What's this? Submitted March 21, 2002 Accepted August 14, 2002 B lymphopoiesis is active throughout human life, but there are developmental age related changes Maria Isabel D Rossi, Takafumi Yokota, Kay L Medina, Karla P Garrett, Philip C Comp, Arthur H Schipul, and Paul W Kincade* Immunobiology and Cancer Program, Oklahoma Medical Research Foundation, Oklahoma City, OK, USA Department of Medicine, University of Oklahoma Health Sciences Center, Oklahoma City, OK, USA St. Anthony Hospital, Oklahoma City, OK, USA * Corresponding author; email: kincade{at}omrf.ouhsc.edu. This study addressed several questions concerning age related changes in human B lymphopoiesis. The relative abundance of pro-B, pre-B, immature, naive and mature B cells among the CD19+ lymphocyte fraction of human bone marrow was found not to change appreciably over the interval between 24 and 88 years of age. Moreover, proliferation of pro-B and large pre-B cells in adult marrow equaled that observed with fetal marrow specimens. Exceptionally low numbers of lymphocyte precursors were found in some marrow samples and the values obtained were used to determine parameters that best reflect B lymphopoiesis. Cord blood always contained higher incidences of functional B lineage lymphocyte precursors than adult cells. However, sorted CD34+ Lin- CD10+ progenitors from cord blood and adult marrow had equivalent potential for differentiation in culture and notable age related changes were found in more primitive subsets. A recently described subset of CD34+ CD38- CD7+ cord blood cells had no exact counterpart in adult marrow. That is, all adult CD34+ Lin- CD7+ CD10- cells expressed CD38, displayed less CD45RA and had little B lineage differentiation potential. The CD7+ fractions in either site contained progenitors for erythroid and NK lineages and ones sorted from bone marrow expressed high levels of transcripts for the CD122 IL-2/IL-15 receptor required by NK lineage precursors. Dramatic changes in human B lymphopoiesis occur early in life and more information is required to construct a probable sequence of differentiation events prior to the time of acquisition of CD10. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Frasca, A. M. Landin, R. L. Riley, and B. B. Blomberg Mechanisms for Decreased Function of B Cells in Aged Mice and Humans J. Immunol., March 1, 2008; 180(5): 2741 - 2746. [Abstract] [Full Text] [PDF] E. M. Six, D. Bonhomme, M. Monteiro, K. Beldjord, M. Jurkowska, C. Cordier-Garcia, A. Garrigue, L. Dal Cortivo, B. Rocha, A. Fischer, et al. A human postnatal lymphoid progenitor capable of circulating and seeding the thymus J. Exp. Med., December 24, 2007; 204(13): 3085 - 3093. [Abstract] [Full Text] [PDF] M. E. Hystad, J. H. Myklebust, T. H. Bo, E. A. Sivertsen, E. Rian, L. Forfang, E. Munthe, A. Rosenwald, M. Chiorazzi, I. Jonassen, et al. 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