SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on December 12, 2002; DOI 10.1182/blood-2002-04-1019. This Article Full Text (PDF) All Versions of this Article: 2002-04-1019v1 101/8/3257    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Borjesson, D. L Articles by Barthold, S. W Search for Related Content PubMed PubMed Citation Articles by Borjesson, D. L Articles by Barthold, S. W Social Bookmarking                   What's this? Submitted April 9, 2002 Accepted November 25, 2002 Roles of Neutrophil 2 Integrins in Kinetics of Bacteremia, Extravasation and Tick Acquisition of Anaplasma phagocytophila in Mice Dori L Borjesson, Scott I Simon, Emir Hodzic, Hilde E DeCock, Christie M Ballantyne, and Stephen W Barthold* Veterinary Diagnostic Medicine, University of Minnesota, St. Paul, MN, USA; Center for Comparative Medicine, University of California, Davis, Davis, CA, USA Biomedical Engineering, University of California, Davis, Davis, CA, USA Center for Comparative Medicine, University of California, Davis, Davis, CA, USA Veterinary Medicine: Pathology, Microbiology, Immunology, University of California, Davis, Davis, CA, USA Medicine and Pediatrics, Baylor College of Medicine, Houston, TX, USA * Corresponding author; email: swbarthold{at}ucdavis.edu. Tick saliva contains anti-inflammatory and immunosuppressive substances that facilitate bloodfeeding and enhance tick-vectored pathogen transmission, including A. phagocytophila, an etiologic agent of granulocytic ehrlichiosis. As such, inflammation at a tick-feeding site is strikingly different than that typically observed at other sites of inflammation. Upregulation of CD11b/CD18 occurs in host granulocytes following interaction or infection with A. phagocytophila, and the absence of CD11b/CD18 results in early increases in bacteremia. We hypothesized that 2 integrin-dependent infection kinetics and leukocyte extravasation are important determinants of neutrophil trafficking to, and pathogen acquisition at tick-feeding sites. A. phagocytophila infection kinetics were evaluated in CD11a/CD18, CD11b/CD18 and CD18 knockout mice using quantitative PCR of blood, ticks and skin biopsies in conjunction with histopathology. A marked increase in the rate of A. phagocytophila infection of neutrophils and pathogen burden in blood followed tick-feeding. Infection kinetics were modified by 2 integrin expression and systemic neutrophil counts. Significant neutrophil-pathogen trafficking was observed to both suture and tick sites. Despite the prominent role for 2 integrins in neutrophil arrest in flowing blood, successful pathogen acquisition by ticks occured in the absence of 2 integrins. Establishment of feeding pools that rely less on leukocyte trafficking and more on small hemorrhages may explain the ready amplification of A. phagocytophila DNA from ticks infested on CD11/CD18 deficient mouse strains. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. L. Johns, K. C. MacNamara, N. J. Walker, G. M. Winslow, and D. L. Borjesson Infection with Anaplasma phagocytophilum Induces Multilineage Alterations in Hematopoietic Progenitor Cells and Peripheral Blood Cells Infect. Immun., September 1, 2009; 77(9): 4070 - 4080. [Abstract] [Full Text] [PDF] U. Blas-Machado, J. de la Fuente, E. F. Blouin, C. Almazan, K. M. Kocan, and J. V. Mysore Experimental Infection of C3H/HeJ Mice with the NY18 Isolate of Anaplasma phagocytophilum Vet. Pathol., January 1, 2007; 44(1): 64 - 73. [Abstract] [Full Text] [PDF] D. L. Borjesson, S. D. Kobayashi, A. R. Whitney, J. M. Voyich, C. M. Argue, and F. R. DeLeo Insights into Pathogen Immune Evasion Mechanisms: Anaplasma phagocytophilum Fails to Induce an Apoptosis Differentiation Program in Human Neutrophils J. Immunol., May 15, 2005; 174(10): 6364 - 6372. [Abstract] [Full Text] [PDF] T. T. Brandhorst, M. Wuthrich, B. Finkel-Jimenez, T. Warner, and B. S. Klein Exploiting Type 3 Complement Receptor for TNF-{alpha} Suppression, Immune Evasion, and Progressive Pulmonary Fungal Infection J. Immunol., December 15, 2004; 173(12): 7444 - 7453. [Abstract] [Full Text] [PDF] H. Wu, J. R. Rodgers, X.-Y. D. Perrard, J. L. Perrard, J. E. Prince, Y. Abe, B. K. Davis, G. Dietsch, C. W. Smith, and C. M. Ballantyne Deficiency of CD11b or CD11d Results in Reduced Staphylococcal Enterotoxin-Induced T Cell Response and T Cell Phenotypic Changes J. Immunol., July 1, 2004; 173(1): 297 - 306. [Abstract] [Full Text] [PDF] J. D. van Buul and P. L. Hordijk Signaling in Leukocyte Transendothelial Migration Arterioscler Thromb Vasc Biol, May 1, 2004; 24(5): 824 - 833. [Abstract] [Full Text]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020