|
|
Prepublished online as a Blood First Edition Paper on December 19, 2002; DOI 10.1182/blood-2002-04-1039.
Submitted April 4, 2002
Accepted November 27, 2002
Regulation of neutrophil and eosinophil secondary granule gene expression by transcription factors C/EBP and PU.1
Adrian F Gombart*, Scott H Kwok, Karen L Anderson, Yuji Yamaguchi, Bruce E Torbett, and H. Phillip Koeffler
Division of Hematology/Oncology, Cedars-Sinai Medical Center, Burns & Allen Research Institute, UCLA School of Medicine, Los Angeles, CA, USA
Departments of Molecular and Experimental Medicine and Immunology, The Scripps Research Institute, La Jolla, CA, USA
Center for Sleep Respiratory Disorders at Fukoka, Fukoka, Japan
* Corresponding author; email: gombarta{at}csmc.edu.
In the bone marrow of C/EBP -/- mice, expression of neutrophil secondary and tertiary granule mRNAs is absent for lactoferrin (LF), neutrophil gelatinase (NG), murine cathelin-like protein (MCLP) and the cathelin B9; and severely reduced for neutrophil collagenase (NC) and neutrophil gelatinase-associated lipocalin (NGAL). Also, expression of eosinophil granule genes, major basic protein (MBP) and eosinphil peroxidase (EPX) is absent. These mice express C/EBP , C/EBP and C/EBP in the bone marrow at levels similar to their wild type counterparts suggesting a lack of functional redundancy among the family in vivo. Stable inducible expression of C/EBPand C/EBP in the murine fibroblast cell line NIH 3T3 activated expression of mRNAs for B9, MCLP, NC and NGAL, but not LF. In transient transfections of C/EBP and C/EBP, B9 was strongly induced with weaker induction of the other genes. The C/EBP and C/EBP proteins weakly induced B9 expression, but C/EBP induced NC expression more efficiently than the other C/EBPs. The expression of MBP was inefficiently induced by C/EBP alone and weakly induced with C/EBP and GATA-1, but the addition of PU.1 resulted in a striking cooperative induction of MBP in NIH 3T3 cells. Mutation of a predicted PU.1 site in the human MBP promoter-luciferase reporter construct abrogated the response to PU.1. Gel shift analysis demonstrated binding of PU.1 to this site. MBP and EPX mRNAs were absent in a PU.1-null myeloid cell line established from the embryonic liver of PU.1-/- mice. Restitution of PU.1 gene expression restored MBP and EPX gene expression. This study demonstrates that C/EBP is essential and sufficient for the expression of a particular subset of neutrophil secondary granule genes. Furthermore, it indicates the importance of PU.1 in the cooperative activation of eosinophil granule genes.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
L. Laricchia-Robbio, K. Premanand, C. R. Rinaldi, and G. Nucifora
EVI1 Impairs Myelopoiesis by Deregulation of PU.1 Function
Cancer Res.,
February 15, 2009;
69(4):
1633 - 1642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Bedi, J. Du, A. K. Sharma, I. Gomes, and S. J. Ackerman
Human C/EBP-{epsilon} activator and repressor isoforms differentially reprogram myeloid lineage commitment and differentiation
Blood,
January 8, 2009;
113(2):
317 - 327.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Yoshida, H. Ichikawa, Y. Tagata, T. Katsumoto, K. Ohnishi, Y. Akao, T. Naoe, P. P. Pandolfi, and I. Kitabayashi
PML-Retinoic Acid Receptor {alpha} Inhibits PML IV Enhancement of PU.1-Induced C/EBP{varepsilon} Expression in Myeloid Differentiation
Mol. Cell. Biol.,
August 15, 2007;
27(16):
5819 - 5834.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. D. Dyer, M. Czapiga, B. Foster, P. S. Foster, E. M. Kang, C. M. Lappas, J. M. Moser, N. Naumann, C. M. Percopo, S. J. Siegel, et al.
Eosinophils from Lineage-Ablated {Delta}dblGATA Bone Marrow Progenitors: The dblGATA Enhancer in the Promoter of GATA-1 Is Not Essential for Differentiation Ex Vivo
J. Immunol.,
August 1, 2007;
179(3):
1693 - 1699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Wagner, C. J. Christensen, D. M. Dunn, G. J. Spangrude, A. Georgelas, L. Kelley, M. S. Esplin, R. B. Weiss, and G. J. Gleich
EGO, a novel, noncoding RNA gene, regulates eosinophil granule protein transcript expression
Blood,
June 15, 2007;
109(12):
5191 - 5198.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, J. Grewal, and H. P. Koeffler
ATF4 differentially regulates transcriptional activation of myeloid-specific genes by C/EBP{epsilon} and C/EBP{alpha}
J. Leukoc. Biol.,
June 1, 2007;
81(6):
1535 - 1547.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. M. Chumakov, A. Silla, E. A. Williamson, and H. P. Koeffler
Modulation of DNA binding properties of CCAAT/enhancer binding protein epsilon by heterodimer formation and interactions with NFkappaB pathway
Blood,
May 15, 2007;
109(10):
4209 - 4219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-J. Lee, L. C. Jones, N. A. Timchenko, D. Perrotti, D. G. Tenen, and S. C. Kogan
CCAAT/enhancer binding proteins alpha and epsilon cooperate with all-trans retinoic acid in therapy but differ in their antileukemic activities
Blood,
October 1, 2006;
108(7):
2416 - 2419.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. S. Hale, T. J. Dahlem, R. L. Margraf, I. Debnath, J. J. Weis, and J. H. Weis
Transcriptional control of Pactolus: evidence of a negative control region and comparison with its evolutionary paralogue, CD18 ({beta}2 integrin)
J. Leukoc. Biol.,
August 1, 2006;
80(2):
383 - 398.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Takeda, C. Goolsby, and N. R. Yaseen
NUP98-HOXA9 Induces Long-term Proliferation and Blocks Differentiation of Primary Human CD34+ Hematopoietic Cells.
Cancer Res.,
July 1, 2006;
66(13):
6628 - 6637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lennartsson, K. Vidovic, M. B. Pass, J. B. Cowland, and U. Gullberg
All-trans retinoic acid-induced expression of bactericidal/permeability-increasing protein (BPI) in human myeloid cells correlates to binding of C/EBP{beta} and C/EBP{epsilon} to the BPI promoter
J. Leukoc. Biol.,
July 1, 2006;
80(1):
196 - 203.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. F. Gombart, U. Krug, J. O'Kelly, E. An, V. Vegesna, and H. P. Koeffler
Aberrant expression of neutrophil and macrophage-related genes in a murine model for human neutrophil-specific granule deficiency
J. Leukoc. Biol.,
November 1, 2005;
78(5):
1153 - 1165.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gery, A. F. Gombart, W. S. Yi, C. Koeffler, W.-K. Hofmann, and H. P. Koeffler
Transcription profiling of C/EBP targets identifies Per2 as a gene implicated in myeloid leukemia
Blood,
October 15, 2005;
106(8):
2827 - 2836.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. Williamson, I. K. Williamson, A. M. Chumakov, A. D. Friedman, and H. P. Koeffler
CCAAT/enhancer binding protein {epsilon}: changes in function upon phosphorylation by p38 MAP kinase
Blood,
May 15, 2005;
105(10):
3841 - 3847.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gery, S. Tanosaki, S. Bose, N. Bose, J. Vadgama, and H. P. Koeffler
Down-Regulation and Growth Inhibitory Role of C/EBP{alpha} in Breast Cancer
Clin. Cancer Res.,
May 1, 2005;
11(9):
3184 - 3190.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gery, D. J. Park, P. T. Vuong, D. Y. Chih, N. Lemp, and H. P. Koeffler
Retinoic acid regulates C/EBP homologous protein expression (CHOP), which negatively regulates myeloid target genes
Blood,
December 15, 2004;
104(13):
3911 - 3917.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Back, A. Dierich, C. Bronn, P. Kastner, and S. Chan
PU.1 determines the self-renewal capacity of erythroid progenitor cells
Blood,
May 15, 2004;
103(10):
3615 - 3623.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Shiohara, A. F. Gombart, Y. Sekiguchi, E. Hidaka, S. Ito, T. Yamazaki, H. P. Koeffler, and A. Komiyama
Phenotypic and functional alterations of peripheral blood monocytes in neutrophil-specific granule deficiency
J. Leukoc. Biol.,
February 1, 2004;
75(2):
190 - 197.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gery, A. F. Gombart, Y. K. Fung, and H. P. Koeffler
C/EBP{epsilon} interacts with retinoblastoma and E2F1 during granulopoiesis
Blood,
February 1, 2004;
103(3):
828 - 835.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
