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Prepublished online as a Blood First Edition Paper on May 31, 2002; DOI 10.1182/blood-2002-04-1064. This Article Full Text (PDF) All Versions of this Article: 2002-04-1064v1 100/7/2449    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Kundu, M. Articles by Liu, P. P. Search for Related Content PubMed PubMed Citation Articles by Kundu, M. Articles by Liu, P. P. Social Bookmarking                   What's this? Submitted April 8, 2002 Accepted May 16, 2002 Role of Cbfb in hematopoeisis and perturbations resulting from expression of the leukemogenic fusion gene, Cbfb-MYH11 Mondira Kundu, Amy Chen, Stacie Anderson, Martha Kirby, LiPing Xu, Lucio H Castilla, David Bodine, and Pu Paul Liu* National Human Genome Research Institute, Genetics and Molecular Biology Branch, National Institutes of Health, Bethesda, MD, USA National Human Genome Research Institute, Genetic Diseases Research Branch, National Institutes of Health, Bethesda, MD, USA Programs in Gene Function and Expression, University of Massachusetts Medical School, Worcester, MA, USA * Corresponding author; email: pliu{at}nhgri.nih.gov. CBFß and CBF2 form a heterodimeric transcription factor that plays an important role in hematopoiesis. The genes encoding either CBFß or CBF2 are involved in chromosomal rearrangements in over 30% of cases of acute myeloid leukemia, suggesting that CBFß and CBF2 play important roles in leukemogenesis. Inv(16)(p13;q22) is found in almost all cases of AML M4Eo and results in the fusion of CBFB with MYH11, the gene encoding smooth muscle myosin heavy chain. Mouse embryos heterozygous for a Cbfb-MYH11 knock-in gene lack definitive hematopoiesis, a phenotype shared by Cbfb-/- embryos. In this study we generated a Cbfb-GFP knock-in mouse model to characterize the normal expression pattern of Cbfß in hematopoietic cells. In mid-gestation embryos, Cbfß was expressed in populations enriched for hematopoietic stem cells and progenitors. This population of stem cells and progenitors was not present in mouse embryos heterozygous for the Cbfb-MYH11 knock-in gene. Together, these data suggest that Cbfb-MYH11 blocks embryonic hematopoiesis at the stem/progenitor cell level and that Cbfß is essential for the generation of hematopoietic stem and progenitor cells. In adult mice, Cbfß was expressed in stem and progenitor cells, as well as mature myeloid and lymphoid cells. Although it was expressed in erythroid progenitors, Cbfß was not expressed during the terminal stages of erythropoiesis. Our data indicates that Cbfß is required for myeloid and lymphoid differentiation; but does not play a critical role in erythroid differentiation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Pulsoni, S. Iacobelli, M. Bernardi, M. Borgia, A. Camera, N. Cantore, F. Di Raimondo, P. Fazi, F. Ferrara, F. Leoni, et al. M4 acute myeloid leukemia: the role of eosinophilia and cytogenetics in treatment response and survival. The GIMEMA experience Haematologica, July 1, 2008; 93(7): 1025 - 1032. [Abstract] [Full Text] [PDF] Y.-H. Kuo, R. M. Gerstein, and L. H. Castilla Cbf{beta}-SMMHC impairs differentiation of common lymphoid progenitors and reveals an essential role for RUNX in early B-cell development Blood, February 1, 2008; 111(3): 1543 - 1551. [Abstract] [Full Text] [PDF] L. 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