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Prepublished online as a Blood First Edition Paper on November 14, 2002; DOI 10.1182/blood-2002-04-1076. This Article Full Text (PDF) All Versions of this Article: 2002-04-1076v1 101/6/2215    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Masuya, M. Articles by Ogawa, M. Search for Related Content PubMed PubMed Citation Articles by Masuya, M. Articles by Ogawa, M. Social Bookmarking                   What's this? Submitted April 8, 2002 Accepted October 18, 2002 Hematopoietic origin of glomerular mesangial cells Masahiro Masuya, Christopher J Drake, Paul A Fleming, Christopher M Reilly, Haiqun Zeng, William D Hill, Angeline Martin-Studdard, David C Hess, and Makio Ogawa* Ralph H. Johnson Veterans Affairs Medical Center, Charleston, SC, USA Department of Medicine, Medical University of SC, Charleston, SC, USA Department of Cell Biology and Anatomy, Medical University of SC, Charleston, SC, USA Department of Neurology, Medical College of Georgia, Augusta, GA, USA Department of Anatomy and Cell Biology, Medical College of Georgia, Augusta, GA, USA Department of Veterans Affairs Medical Center, Augusta, GA, USA * Corresponding author; email: ogawam{at}musc.edu. It was recently reported that crude bone marrow cells have the ability to differentiate into glomerular mesangial cells. However, the exact nature of the engrafting cells in the bone marrow was not known. We tested the hypothesis that hematopoietic stem cells are capable of reconstituting the mesangial cells by transplanting a clonal population of cells derived from a single stem cell. We cultured Lin-, Sca-1+, c-kit+, CD34- bone marrow cells from transgenic enhanced green fluorescent protein (EGFP)- mice (C57BL/6-Ly-5.2 background) individually for 1 week in the presence of interleukin-11 and steel factor. We then transplanted viable clones individually into lethally irradiated C57BL/6-Ly-5.1 mice. Two to six months later, kidneys from five recipient mice showing high levels (60-90%) of multilineage hematopoietic reconstitution were examined using differential interference contrast and epifluorescence microscopy. EGFP+ cells with a morphology characteristic of mesangial cells were evident within the glomeruli. Transplantation of 100 non-cultured Lin-, Sca-1+, c-kit+, CD34- bone marrow cells also generated mesangial cells. Cultured EGFP+ glomerular cells from recipient mice contracted in response to angiotensin II. EGFP+ mesangial cells seen in male-to-male transplantation revealed only one Y-chromosome. These data demonstrate that a single hematopoietic stem cell is capable of differentiating into glomerular mesangial cells and that the process does not involve cell fusion. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. C.W. Hung, T. K.F. Shing, S. S.C. Chim, P. C. Yeung, R. W.Y. Chan, K. W. Chik, V. Lee, N. B.Y. Tsui, C.-K. Li, C. S.C. Wong, et al. Presence of Donor-Derived DNA and Cells in the Urine of Sex-Mismatched Hematopoietic Stem Cell Transplant Recipients: Implication for the Transrenal Hypothesis Clin. Chem., April 1, 2009; 55(4): 715 - 722. [Abstract] [Full Text] [PDF] S. E. Quaggin and J. A. 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