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Prepublished online as a Blood First Edition Paper on August 1, 2002; DOI 10.1182/blood-2002-04-1115. This Article Full Text (PDF) All Versions of this Article: 2002-04-1115v1 100/13/4565    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Suto, A. Articles by Iwamoto, I. Search for Related Content PubMed PubMed Citation Articles by Suto, A. Articles by Iwamoto, I. Social Bookmarking                   What's this? Submitted April 19, 2002 Accepted July 20, 2002 Interleukin-21 prevents antigen-induced IgE production by inhibiting germline C transcription of IL-4-stimulated B cells Akira Suto, Hiroshi Nakajima*, Koichi Hirose, Kotaro Suzuki, Shin-ichiro Kagami, Yohei Seto, Aihiro Hoshimoto, Yasushi Saito, Donald C Foster, and Itsuo Iwamoto Internal Medicine II, Chiba University School of Medicine, Chiba, Japan Cytokine Biology, ZymoGenetics Inc., Seattle, WA, USA * Corresponding author; email: nakajimh{at}intmed02.m.chiba-u.ac.jp. Interleukin-21 (IL-21) has recently been identified as a multi-functional cytokine that induces the proliferation of T cells and B cells and differentiation of natural killer cells. To determine whether IL-21 regulates IL-4-mediated immune responses, we examined the effect of IL-21 on antigen-specific IgE production in mice. We also examined the effect of IL-21 on IL-4-induced IgE production from B cells and antigen-induced T helper 2 (Th2) cell differentiation. The in vivo injection of IL-21 prevented antigen-specific IgE but not IgG2a production upon immunization. IL-21 did not affect Th2 cell differentiation or IL-4 production from CD4+ T cells but directly inhibited IL-4-induced IgE production from B cells at single cell levels. Moreover, IL-21 inhibited IL-4-induced germline C transcription in B cells without the inhibition of Stat6 activation. Taken together, these results indicate that IL-21 downregulates IgE production from IL-4-stimulated B cells through the inhibition of germline C transcription and thus suggest that IL-21 may be useful for the treatment of IgE-dependent allergic diseases. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. J. Reece, M. C. Siracusa, T. L. Southard, C. F. Brayton, J. F. Urban Jr., and A. L. Scott Hookworm-Induced Persistent Changes to the Immunological Environment of the Lung Infect. 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