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Prepublished online as a Blood First Edition Paper on May 31, 2002; DOI 10.1182/blood-2002-04-1182. This Article Full Text (PDF) All Versions of this Article: 2002-04-1182v1 100/7/2617    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jabado, N. Articles by Gros, P. Search for Related Content PubMed PubMed Citation Articles by Jabado, N. Articles by Gros, P. Social Bookmarking                   What's this? Submitted April 19, 2002 Accepted May 17, 2002 The iron transporter Nramp2/DMT-1 is associated with the membrane of phagosomes in macrophages and Sertoli cells Nada Jabado, Francois Canonne-Hergaux, Samantha Gruenheid, Virginie Picard, and Philippe Gros* * Corresponding author; email: gros{at}med.mcgill.ca. Nramp2 (DMT 1) is a pH-dependent divalent cation transporter that acts as the transferrin-independent iron uptake system at the intestinal brush border and also transports iron released from transferrin across the membrane of acidified endosomes. In this study, RAW 264.7 macrophages and two independently derived murine Sertoli cells lines, TM4 and 15P-1, were used to further study the subcellular localization of Nramp2/DMT1 in phagocytic cells including possible recruitment to the phagosomal membrane. Nramp2/DMT1 was localized primarily to the EEA1-positive recycling endosome compartment, with some overlapping staining with Lamp1-positive late endosomes. After phagocytosis, immunofluorescence analysis and in vitro biochemical studies using purified Latex bead-containing phagosomes indicated Nramp2/DMT1 recruitment to the membrane of Lamp1, cathepsin D and rab7-positive phagosomes. Nramp2/DMT1 was also found associated with erythrocyte-containing phagosomes in RAW macrophages and with the periphery of sperm-containing phagosomes in Sertoli cells. These results suggest that, as for the macrophage-specific Nramp1 protein, Nramp2/DMT1 may transport divalent metals from the phagosomal space. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: O. Olakanmi, L. S. Schlesinger, and B. E. Britigan Hereditary hemochromatosis results in decreased iron acquisition and growth by Mycobacterium tuberculosis within human macrophages J. Leukoc. Biol., January 1, 2007; 81(1): 195 - 204. [Abstract] [Full Text] [PDF] C. Zhang, S. Yeh, Y.-T. Chen, C.-C. Wu, K.-H. Chuang, H.-Y. Lin, R.-S. Wang, Y.-J. Chang, C. Mendis-Handagama, L. Hu, et al. Oligozoospermia with normal fertility in male mice lacking the androgen receptor in testis peritubular myoid cells PNAS, November 21, 2006; 103(47): 17718 - 17723. [Abstract] [Full Text] [PDF] J. L. Folwell, C. H. Barton, and D. Shepherd Immunolocalisation of the D. melanogaster Nramp homologue Malvolio to gut and Malpighian tubules provides evidence that Malvolio and Nramp2 are orthologous J. Exp. Biol., May 15, 2006; 209(10): 1988 - 1995. [Abstract] [Full Text] [PDF] J. Caron, L. Lariviere, M. Nacache, M. Tam, M. M. Stevenson, C. McKerly, P. Gros, and D. Malo Influence of Slc11a1 on the Outcome of Salmonella enterica Serovar Enteritidis Infection in Mice Is Associated with Th Polarization. Infect. Immun., May 1, 2006; 74(5): 2787 - 2802. [Abstract] [Full Text] [PDF] A. Donovan, C. N. Roy, and N. C. Andrews The Ins and Outs of Iron Homeostasis Physiology, April 1, 2006; 21(2): 115 - 123. [Abstract] [Full Text] [PDF] I. Gillot, C. Jehl-Pietri, P. Gounon, S. Luquet, M. Rassoulzadegan, P. Grimaldi, and F. Vidal Germ cells and fatty acids induce translocation of CD36 scavenger receptor to the plasma membrane of Sertoli cells J. Cell Sci., July 15, 2005; 118(14): 3027 - 3035. [Abstract] [Full Text] [PDF] K. P. Griffin, D. T. Ward, W. Liu, G. Stewart, I. D. Morris, and C. P. Smith Differential expression of divalent metal transporter DMT1 (Slc11a2) in the spermatogenic epithelium of the developing and adult rat testis Am J Physiol Cell Physiol, January 1, 2005; 288(1): C176 - C184. [Abstract] [Full Text] [PDF] N. Touret, N. Martin-Orozco, P. Paroutis, W. Furuya, S. Lam-Yuk-Tseung, J. Forbes, P. Gros, and S. Grinstein Molecular and cellular mechanisms underlying iron transport deficiency in microcytic anemia Blood, September 1, 2004; 104(5): 1526 - 1533. [Abstract] [Full Text] [PDF] S. Ludwiczek, E. Aigner, I. Theurl, and G. Weiss Cytokine-mediated regulation of iron transport in human monocytic cells Blood, May 15, 2003; 101(10): 4148 - 4154. [Abstract] [Full Text] [PDF]

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