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Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-04-1285.
Submitted April 30, 2002
Accepted August 6, 2002
Evidence that the red cell skeleton protein 4.2 interacts with the Rh membrane complex member CD47
Isabelle Mouro-Chanteloup, Jean Delaunay, Pierre Gane, Virginie Nicolas, Mette Johansen, Eric J Brown, Luanne L Peters, Caroline Le Van Kim, Jean Pierre Cartron, and Yves Colin*
Unite 76, INSERM, Paris, France
Unite 473, INSERM, Le Kremlin Bicetre, France
Center for Host/Pathogen Interactions, University of California, San Francisco, CA, USA
The Jackson Laboratory, Bar Harbor, ME, USA
* Corresponding author; email: colin{at}idf.inserm.fr.
Rhnull red cells are characteristically stomato-spherocytic. This and other evidence suggests that the Rh complex represents a major attachment site between the membrane lipid bilayer and the erythroid skeleton. As an attempt to identify the linking protein(s) between the red cell skeleton and the Rh complex, we analysed the expression of Rh, RhAG, CD47, LW and GPB proteins in red cells from patients with hereditary spherocytosis associated with complete protein 4.2 deficiency but normal band 3 [4.2(-)HS]. Flow cytometric and immunoblotting analysis revealed a severe reduction of CD47 (up to 80%) and a slower mobility of RhAG on SDS-PAGE, possibly reflecting an overglycosylation state. Unexpectedly, 4.2-/- mice, which are anemic, displayed a normal red cell expression of CD47 and RhAG. These results suggest that human protein 4.2, through interaction with CD47, is involved in the skeleton linkage and/or membrane translocation of the Rh complex. However, these potential role(s) of protein 4.2 might be not conserved across species. Finally, the absence or low expression of red cell CD47 in CD47-/- mice and in some humans carrying RHCE gene variants (D--, D.. and RN), respectively, had no detectable effect on protein 4.2 and RhAG expression. Since, these cells are morphologically normal with no sign of hemolysis, it is assumed that CD47 deficiency per se is not responsible of the cell shape abnormalities and of the compensated hemolytic anemia typical of 4.2(-) and Rhnull red cells.
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