|
|
Prepublished online as a Blood First Edition Paper on August 8, 2002; DOI 10.1182/blood-2002-04-1286.
Submitted May 6, 2002
Accepted July 26, 2002
Clinical impact of the differentiation profile assessed by immunophenotyping in patients with diffuse large B-cell lymphoma
Lluis Colomo, Armando Lopez-Guillermo*, Maria Perales, Susana Rives, Antonio Martinez, Francesc Bosch, Dolors Colomer, Brunangelo Falini, Emili Montserrat, and Elias Campo
Department of Pathology, Hospital Clinic, Institut de Recerca Biomedica August Pi i Sunyer, University of Barcelona, Barcelona, Spain
Department of Hematology, Hospital Clinic, Institut de Recerca Biomedica August Pi i Sunyer, University of Barcelona, Barcelona, Spain
Institute of Hematology, Policlinico Monteluce, University of Perugia, Perugia, Italy
* Corresponding author; email: alopezg{at}clinic.ub.es.
To analyze the relationship between immunophenotyping profile and main clinicopathological features and outcome in diffuse large B-cell lymphoma (DLBCL), 128 patients (59M/69F; median age 65 years) consecutively diagnosed with de novo DLBCL in a single institution were studied. All cases were immunostained with CD20, CD79a, CD5, CD10, bcl-6, MUM-1, CD138, bcl-2, p53, p27 and Ki-67 antibodies. Four different immunophenotyping profiles were distinguished according to the pattern of differentiation: germinal-center CD10+ [GC-CD10+] (CD10+/Bcl-6+/MUM1-/ CD138-), germinal-center CD10- [GC-CD10-] (CD10-/Bcl-6+/ MUM1-/CD138-), post-germinal-center [pGC] (CD10-/Bcl-6+/ MUM1+/CD138-), and plasmablastic (CD10-/Bcl-6-/MUM1+/CD138+). Bcl-2 rearrangement was studied by PCR in 57 cases. The single antigen expression was: CD5, 2%; CD10, 21%; bcl-6, 72%; MUM-1, 54%; CD138, 2%; bcl-2, 59%; p53, 28%; p27, 40%. The distribution according to differentiation profiles was: GC-CD10+, 24 cases (19%), GC-CD10-, 30 (24%); pGC, 60 (47.5%); plasmablastic, 2 (1.5%); other pattern, 12. pGC profile was associated with primary nodal presentation and immunoblastic morphology, whereas GC-CD10+ tumors showed disseminated disease, centroblastic morphology, bcl-2 rearrangement and lower Ki-67 proliferative index. GC-CD10- patients more often presented with primary extranodal origin, early stage, normal LDH levels and low or low-intermediate IPI than the others. However, no significant difference was found in terms of response or overall survival (OS) according to these profiles. Bcl-2 expression was associated with advanced stage, high or high-intermediate IPI and poor OS. Bcl-2 expression maintained predictive value in multivariate analysis, with stage and LDH. In conclusion, differentiation profile was associated with particular clinicopathological features, but was not essential to predict outcome in DLBCL patients.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Copie-Bergman, P. Gaulard, K. Leroy, J. Briere, M. Baia, J.-P. Jais, G. A. Salles, F. Berger, C. Haioun, H. Tilly, et al.
Immuno-Fluorescence In Situ Hybridization Index Predicts Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With R-CHOP: A GELA Study
J. Clin. Oncol.,
November 20, 2009;
27(33):
5573 - 5579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H.-W. Bernd, M. Ziepert, C. Thorns, W. Klapper, H.-H. Wacker, M. Hummel, H. Stein, M.-L. Hansmann, G. Ott, A. Rosenwald, et al.
Loss of HLA-DR expression and immunoblastic morphology predict adverse outcome in diffuse large B-cell lymphoma - analyses of cases from two prospective randomized clinical trials
Haematologica,
November 1, 2009;
94(11):
1569 - 1580.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E C Obermann, M Csato, S Dirnhofer, and A Tzankov
BCL2 gene aberration as an IPI-independent marker for poor outcome in non-germinal-centre diffuse large B cell lymphoma
J. Clin. Pathol.,
October 1, 2009;
62(10):
903 - 907.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. W.L. Choi, D. D. Weisenburger, T. C. Greiner, M. A. Piris, A. H. Banham, J. Delabie, R. M. Braziel, H. Geng, J. Iqbal, G. Lenz, et al.
A New Immunostain Algorithm Classifies Diffuse Large B-Cell Lymphoma into Molecular Subtypes with High Accuracy
Clin. Cancer Res.,
September 1, 2009;
15(17):
5494 - 5502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D de Jong, W Xie, A Rosenwald, M Chhanabhai, P Gaulard, W Klapper, A Lee, B Sander, C Thorns, E Campo, et al.
Immunohistochemical prognostic markers in diffuse large B-cell lymphoma: validation of tissue microarray as a prerequisite for broad clinical applications (a study from the Lunenburg Lymphoma Biomarker Consortium)
J. Clin. Pathol.,
February 1, 2009;
62(2):
128 - 138.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Amara, M. Trimeche, S. Ziadi, A. Laatiri, M. Hachana, and S. Korbi
Prognostic significance of aberrant promoter hypermethylation of CpG islands in patients with diffuse large B-cell lymphomas
Ann. Onc.,
October 1, 2008;
19(10):
1774 - 1786.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Garcia-Herrera, L. Colomo, M. Camos, J. Carreras, O. Balague, A. Martinez, A. Lopez-Guillermo, T. Estrach, and E. Campo
Primary Cutaneous Small/Medium CD4+ T-Cell Lymphomas: A Heterogeneous Group of Tumors With Different Clinicopathologic Features and Outcome
J. Clin. Oncol.,
July 10, 2008;
26(20):
3364 - 3371.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nakamura, H. Ye, C. M. Bacon, A. Goatly, H. Liu, L. Kerr, A. H. Banham, B. Streubel, T. Yao, M. Tsuneyoshi, et al.
Translocations Involving the Immunoglobulin Heavy Chain Gene Locus Predict Better Survival in Gastric Diffuse Large B-Cell Lymphoma
Clin. Cancer Res.,
May 15, 2008;
14(10):
3002 - 3010.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dierlamm, E. M. Murga Penas, S. Bentink, S. Wessendorf, H. Berger, M. Hummel, W. Klapper, D. Lenze, A. Rosenwald, E. Haralambieva, et al.
Gain of chromosome region 18q21 including the MALT1 gene is associated with the activated B-cell-like gene expression subtype and increased BCL2 gene dosage and protein expression in diffuse large B-cell lymphoma
Haematologica,
May 1, 2008;
93(5):
688 - 696.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Morel, P. Gaulard, C. Gisselbrecht, C. Ferme, G. Salles, H. Tilly, J. Briere, M. C. Copin, P. Lederlin, O. Hermine, et al.
Autologous stem-cell transplantation as consolidation therapy for diffuse large B-cell lymphoma patients with overexpression of bcl-2 protein. Results of the Groupe d'Etude des Lymphomes de l'Adulte (GELA) trial LNH98-B2
Ann. Onc.,
March 1, 2008;
19(3):
560 - 565.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Natkunam, P. Farinha, E. D. Hsi, C. P. Hans, R. Tibshirani, L. H. Sehn, J. M. Connors, D. Gratzinger, M. Rosado, S. Zhao, et al.
LMO2 Protein Expression Predicts Survival in Patients With Diffuse Large B-Cell Lymphoma Treated With Anthracycline-Based Chemotherapy With and Without Rituximab
J. Clin. Oncol.,
January 20, 2008;
26(3):
447 - 454.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B Lofstrom, C Backlin, C Sundstrom, A Ekbom, and I E Lundberg
A closer look at non-Hodgkin's lymphoma cases in a national Swedish systemic lupus erythematosus cohort: a nested case-control study
Ann Rheum Dis,
December 1, 2007;
66(12):
1627 - 1632.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Ramos-Vara, M. A. Miller, and V. E. O. Valli
Immunohistochemical Detection of Multiple Myeloma 1/Interferon Regulatory Factor 4 (MUM1/IRF-4) in Canine Plasmacytoma: Comparison with CD79a and CD20
Vet. Pathol.,
November 1, 2007;
44(6):
875 - 884.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Dupuis, P. Gaulard, F. Hemery, E. Itti, C. Gisselbrecht, A. Rahmouni, C. Copie-Bergman, J. Briere, T. E. Gnaoui, I. Gaillard, et al.
Respective prognostic values of germinal center phenotype and early 18fluorodeoxyglucose-positron emission tomography scanning in previously untreated patients with diffuse large B-cell lymphoma
Haematologica,
June 1, 2007;
92(6):
778 - 783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Nyman, M. Adde, M.-L. Karjalainen-Lindsberg, M. Taskinen, M. Berglund, R.-M. Amini, C. Blomqvist, G. Enblad, and S. Leppa
Prognostic impact of immunohistochemically defined germinal center phenotype in diffuse large B-cell lymphoma patients treated with immunochemotherapy
Blood,
June 1, 2007;
109(11):
4930 - 4935.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H Veelken, S Vik Dannheim, J Schulte Moenting, U. Martens, J Finke, and A Schmitt-Graeff
Immunophenotype as prognostic factor for diffuse large B-cell lymphoma in patients undergoing clinical risk-adapted therapy
Ann. Onc.,
May 1, 2007;
18(5):
931 - 939.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. de Jong, A. Rosenwald, M. Chhanabhai, P. Gaulard, W. Klapper, A. Lee, B. Sander, C. Thorns, E. Campo, T. Molina, et al.
Immunohistochemical Prognostic Markers in Diffuse Large B-Cell Lymphoma: Validation of Tissue Microarray As a Prerequisite for Broad Clinical Applications--A Study From the Lunenburg Lymphoma Biomarker Consortium
J. Clin. Oncol.,
March 1, 2007;
25(7):
805 - 812.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Natkunam, S. Zhao, D. Y. Mason, J. Chen, B. Taidi, M. Jones, A. S. Hammer, S. Hamilton Dutoit, I. S. Lossos, and R. Levy
The oncoprotein LMO2 is expressed in normal germinal-center B cells and in human B-cell lymphomas
Blood,
February 15, 2007;
109(4):
1636 - 1642.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. van Imhoff, E.-J. G. Boerma, B. van der Holt, E. Schuuring, L. F. Verdonck, H. C. Kluin-Nelemans, and P. M. Kluin
Prognostic Impact of Germinal Center-Associated Proteins and Chromosomal Breakpoints in Poor-Risk Diffuse Large B-Cell Lymphoma
J. Clin. Oncol.,
September 1, 2006;
24(25):
4135 - 4142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. N. Winter, E. A. Weller, S. J. Horning, M. Krajewska, D. Variakojis, T. M. Habermann, R. I. Fisher, P. J. Kurtin, W. R. Macon, M. Chhanabhai, et al.
Prognostic significance of Bcl-6 protein expression in DLBCL treated with CHOP or R-CHOP: a prospective correlative study
Blood,
June 1, 2006;
107(11):
4207 - 4213.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Oschlies, W. Klapper, M. Zimmermann, M. Krams, H.-H. Wacker, B. Burkhardt, L. Harder, R. Siebert, A. Reiter, and R. Parwaresch
Diffuse large B-cell lymphoma in pediatric patients belongs predominantly to the germinal-center type B-cell lymphomas: a clinicopathologic analysis of cases included in the German BFM (Berlin-Frankfurt-Munster) Multicenter Trial
Blood,
May 15, 2006;
107(10):
4047 - 4052.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Lossos and D. Morgensztern
Prognostic Biomarkers in Diffuse Large B-Cell Lymphoma
J. Clin. Oncol.,
February 20, 2006;
24(6):
995 - 1007.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Iqbal, V. T. Neppalli, G. Wright, B. J. Dave, D. E. Horsman, A. Rosenwald, J. Lynch, C. P. Hans, D. D. Weisenburger, T. C. Greiner, et al.
BCL2 Expression Is a Prognostic Marker for the Activated B-Cell-Like Type of Diffuse Large B-Cell Lymphoma
J. Clin. Oncol.,
February 20, 2006;
24(6):
961 - 968.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-H. Lin, K.-T. Kuo, S.-S. Chuang, S.-H. Kuo, J. H. Chang, K.-C. Chang, H.-C. Hsu, H.-F. Tien, and A.-L. Cheng
Comparison of the Expression and Prognostic Significance of Differentiation Markers between Diffuse Large B-Cell Lymphoma of Central Nervous System Origin and Peripheral Nodal Origin
Clin. Cancer Res.,
February 15, 2006;
12(4):
1152 - 1156.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. H. Sehn
Optimal Use of Prognostic Factors in Non-Hodgkin Lymphoma
Hematology,
January 1, 2006;
2006(1):
295 - 302.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Camilleri-Broet, E. Criniere, P. Broet, V. Delwail, K. Mokhtari, A. Moreau, M. Kujas, M. Raphael, W. Iraqi, C. Sautes-Fridman, et al.
A uniform activated B-cell-like immunophenotype might explain the poor prognosis of primary central nervous system lymphomas: analysis of 83 cases
Blood,
January 1, 2006;
107(1):
190 - 196.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. De Paepe, R. Achten, G. Verhoef, I. Wlodarska, M. Stul, V. Vanhentenrijk, M. Praet, and C. De Wolf-Peeters
Large Cleaved and Immunoblastic Lymphoma May Represent Two Distinct Clinicopathologic Entities Within the Group of Diffuse Large B-Cell Lymphomas
J. Clin. Oncol.,
October 1, 2005;
23(28):
7060 - 7068.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Hoffmann, M. Tiemann, C. Schrader, D. Janssen, E. Wolf, M. Vierbuchen, R. Parwaresch, K. Ernestus, A. Plettenberg, A. Stoehr, et al.
AIDS-related B-cell lymphoma (ARL): correlation of prognosis with differentiation profiles assessed by immunophenotyping
Blood,
September 1, 2005;
106(5):
1762 - 1769.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Natkunam, I. S. Lossos, B. Taidi, S. Zhao, X. Lu, F. Ding, A. S. Hammer, T. Marafioti, G. E. Byrne Jr, S. Levy, et al.
Expression of the human germinal center-associated lymphoma (HGAL) protein, a new marker of germinal center B-cell derivation
Blood,
May 15, 2005;
105(10):
3979 - 3986.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Lopez-Guillermo, L. Colomo, M. Jimenez, F. Bosch, N. Villamor, L. Arenillas, A. Muntanola, S. Montoto, E. Gine, D. Colomer, et al.
Diffuse Large B-Cell Lymphoma: Clinical and Biological Characterization and Outcome According to the Nodal or Extranodal Primary Origin
J. Clin. Oncol.,
April 20, 2005;
23(12):
2797 - 2804.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Bea, L. Colomo, A. Lopez-Guillermo, I. Salaverria, X. Puig, M. Pinyol, S. Rives, E. Montserrat, and E. Campo
Clinicopathologic Significance and Prognostic Value of Chromosomal Imbalances in Diffuse Large B-Cell Lymphomas
J. Clin. Oncol.,
September 1, 2004;
22(17):
3498 - 3506.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Lossos, D. K. Czerwinski, A. A. Alizadeh, M. A. Wechser, R. Tibshirani, D. Botstein, and R. Levy
Prediction of Survival in Diffuse Large-B-Cell Lymphoma Based on the Expression of Six Genes
N. Engl. J. Med.,
April 29, 2004;
350(18):
1828 - 1837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. P. Hans, D. D. Weisenburger, T. C. Greiner, R. D. Gascoyne, J. Delabie, G. Ott, H. K. Muller-Hermelink, E. Campo, R. M. Braziel, E. S. Jaffe, et al.
Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray
Blood,
January 1, 2004;
103(1):
275 - 282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
