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Prepublished online as a Blood First Edition Paper on September 12, 2002; DOI 10.1182/blood-2002-05-1344. This Article Full Text (PDF) All Versions of this Article: 2002-05-1344v1 101/3/856    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nilsson, S. K Articles by Simmons, P. J Search for Related Content PubMed PubMed Citation Articles by Nilsson, S. K Articles by Simmons, P. J Social Bookmarking                   What's this? Submitted May 8, 2002 Accepted August 27, 2002 Hyaluronan is synthesized by primitive hemopoietic cells, participates in their lodgment at the endosteum following transplantation, and is involved in the regulation of their proliferation and differentiation in vitro Susan K Nilsson*, David N Haylock, Hayley M Johnston, Teresa Occhiodoro, Tracey J Brown, and Paul J Simmons Stem Cell Biology Laboratory, Peter MacCallum Cancer Institute, Melbourne, VIC, Australia Department of Biochemistry and Molecular Biology, Monash University, Melbourne, VIC, Australia * Corresponding author; email: s.nilsson{at}pmci.unimelb.edu.au. The localization of adult hemopoiesis to the marrow involves developmentally regulated interactions between hemopoietic stem cells and the stromal cell mediated hemopoietic microenvironment. Although primitive hemopoietic cells exhibit a broad repertoire of adhesion molecules, little is known about the molecules influencing the site of cell lodgment within the marrow following transplantation. However, our recent studies indicate that hierarchically dependent patterns of migration of transplanted hemopoietic cells result in the retention of primitive cells within the endosteal and lineage committed cells in the central marrow regions. Herein, we now demonstrate that these two sub-populations exhibit a striking difference in the expression of a cell surface adhesion molecule, with populations enriched for murine and human haemopoietic stem cells expressing the carbohydrate hyaluronic acid (HA). Furthermore, the presence of this glycosaminoglycan appears critical for the spatial distribution of transplanted stem cells in vivo. In addition, we also demonstrate that the binding of HA by a surrogate ligand results in marked inhibition of primitive hemopoietic cell proliferation and granulocyte differentiation. Collectively, these data describe an important, yet previously unrecognised role for HA in the biology of primitive hemopoietic progenitor cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: E. G. Contreras, M. Gaete, N. Sanchez, H. Carrasco, and J. Larrain Early requirement of Hyaluronan for tail regeneration in Xenopus tadpoles Development, September 1, 2009; 136(17): 2987 - 2996. [Abstract] [Full Text] [PDF] J. Grassinger, D. N. Haylock, M. J. Storan, G. O. Haines, B. Williams, G. A. Whitty, A. R. Vinson, C. L. Be, S. Li, E. S. Sorensen, et al. 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