SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on August 8, 2002; DOI 10.1182/blood-2002-05-1359. This Article Full Text (PDF) All Versions of this Article: 2002-05-1359v1 100/12/3960    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Horn, P. A Articles by Kiem, H.-P. Search for Related Content PubMed PubMed Citation Articles by Horn, P. A Articles by Kiem, H.-P. Social Bookmarking                   What's this? Submitted May 9, 2002 Accepted July 4, 2002 Highly efficient gene transfer into baboon marrow repopulating cells using GALV-pseudotype oncoretroviral vectors produced by human packaging cells Peter A Horn, Max S Topp, Julia C Morris, Stanley R Riddell, and Hans-Peter Kiem* Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA; Department of Medicine, University of Washington, Seattle, WA, USA * Corresponding author; email: hkiem{at}fhcrc.org. Vector-containing medium harvested from murine packaging cell lines has been shown to contain factors which can negatively influence the transduction and maintenance of hematopoietic stem cells. Thus, we generated a human packaging cell line with a gibbon ape leukemia virus pseudotype (Phoenix-GALV), and we evaluated vectors produced by Phoenix-GALV for their ability to transduce hematopoietic progenitor/stem cells. In three baboons, we used a competitive repopulation assay to directly compare GALV-pseudotype retrovirus vectors produced by either Phoenix-GALV or by the NIH 3T3-derived packaging cell line, PG13. In three additional baboons we compared Phoenix-GALV-derived vectors to more recently developed lentiviral vectors. Gene transfer efficiency into hematopoietic repopulating cells was assessed by evaluating the number of genetically modified peripheral blood and marrow cells using flow cytometry and real-time PCR. Transduction efficiency into hematopoietic repopulating cells was significantly higher using the Phoenix-GALV-derived vector as compared to the PG13-derived vectors or lentiviral vectors, with stable transduction levels up to 25%. Two animals were followed for more than 1 year. Flow cytometric analysis of hematopoietic subpopulations in these animals revealed transgene expression in CD13+ granulocytes, CD20+ B lymphocytes, CD3+ T lymphocytes, CD61+ platelets, as well as red blood cells, indicating multilineage engraftment of cells transduced by Phoenix-GALV-pseudotype vectors. In addition, transduction efficiency into human CD34+ cells was significantly higher than transduction of baboon CD34+ cells, suggesting that Phoenix-GALV-derived oncoretroviral vectors may be even more efficient in human stem cell gene therapy applications. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: G. D. Trobridge, B. C. Beard, C. Gooch, M. Wohlfahrt, P. Olsen, J. Fletcher, P. Malik, and H.-P. Kiem Efficient transduction of pigtailed macaque hematopoietic repopulating cells with HIV-based lentiviral vectors Blood, June 15, 2008; 111(12): 5537 - 5543. [Abstract] [Full Text] [PDF] B. E. Shepherd, H.-P. Kiem, P. M. Lansdorp, C. E. Dunbar, G. Aubert, A. LaRochelle, R. Seggewiss, P. Guttorp, and J. L. Abkowitz Hematopoietic stem-cell behavior in nonhuman primates Blood, September 15, 2007; 110(6): 1806 - 1813. [Abstract] [Full Text] [PDF] E. Adamopoulou, J. Diekmann, E. Tolosa, G. Kuntz, H. Einsele, H.-G. Rammensee, and M. S. Topp Human CD4+ T Cells Displaying Viral Epitopes Elicit a Functional Virus-Specific Memory CD8+ T Cell Response J. Immunol., May 1, 2007; 178(9): 5465 - 5472. [Abstract] [Full Text] [PDF] T. R. Bauer Jr, M. Hai, L. M. Tuschong, T. H. Burkholder, Y.-c. Gu, R. A. Sokolic, C. Ferguson, C. E. Dunbar, and D. D. Hickstein Correction of the disease phenotype in canine leukocyte adhesion deficiency using ex vivo hematopoietic stem cell gene therapy Blood, November 15, 2006; 108(10): 3313 - 3320. [Abstract] [Full Text] [PDF] T. Neff, B. C. Beard, and H.-P. Kiem Survival of the fittest: in vivo selection and stem cell gene therapy Blood, March 1, 2006; 107(5): 1751 - 1760. [Abstract] [Full Text] [PDF] B. Liu, J. Daviau, C. N. Nichols, and D. S. Strayer In vivo gene transfer into rat bone marrow progenitor cells using rSV40 viral vectors Blood, October 15, 2005; 106(8): 2655 - 2662. [Abstract] [Full Text] [PDF] T. Kalina, H. Lu, Z. Zhao, E. Blewett, D. P. Dittmer, J. Randolph-Habecker, D. G. Maloney, R. G. Andrews, H.-P. Kiem, and J. Storek De novo generation of CD4 T cells against viruses present in the host during immune reconstitution Blood, March 15, 2005; 105(6): 2410 - 2414. [Abstract] [Full Text] [PDF] R. E. Donahue, I. S. Y. Chen, J. C. Morris, and H.-P. Kiem Transgene-specific tolerance versus immune response Blood, September 1, 2004; 104(5): 1578 - 1579. [Full Text] [PDF] H. Hanawa, P. Hematti, K. Keyvanfar, M. E. Metzger, A. Krouse, R. E. Donahue, S. Kepes, J. Gray, C. E. Dunbar, D. A. Persons, et al. Efficient gene transfer into rhesus repopulating hematopoietic stem cells using a simian immunodeficiency virus-based lentiviral vector system Blood, June 1, 2004; 103(11): 4062 - 4069. [Abstract] [Full Text] [PDF] P. A. Horn, K. A. Keyser, L. J. Peterson, T. Neff, B. M. Thomasson, J. Thompson, and H.-P. Kiem Efficient lentiviral gene transfer to canine repopulating cells using an overnight transduction protocol Blood, May 15, 2004; 103(10): 3710 - 3716. [Abstract] [Full Text] [PDF] J. C. Morris, M. Conerly, B. Thomasson, J. Storek, S. R. Riddell, and H.-P. Kiem Induction of cytotoxic T-lymphocyte responses to enhanced green and yellow fluorescent proteins after myeloablative conditioning Blood, January 15, 2004; 103(2): 492 - 499. [Abstract] [Full Text] [PDF] E. Kondo, Y. Akatsuka, K. Kuzushima, K. Tsujimura, S. Asakura, K. Tajima, Y. Kagami, Y. Kodera, M. Tanimoto, Y. Morishima, et al. Identification of novel CTL epitopes of CMV-pp65 presented by a variety of HLA alleles Blood, January 15, 2004; 103(2): 630 - 638. [Abstract] [Full Text] [PDF] P. A. Horn, B. M. Thomasson, B. L. Wood, R. G. Andrews, J. C. Morris, and H.-P. Kiem Distinct hematopoietic stem/progenitor cell populations are responsible for repopulating NOD/SCID mice compared with nonhuman primates Blood, December 15, 2003; 102(13): 4329 - 4335. [Abstract] [Full Text] [PDF] P. Kurre, J. Morris, B. Thomasson, D. B. Kohn, and H.-P. Kiem Scaffold attachment region-containing retrovirus vectors improve long-term proviral expression after transplantation of GFP-modified CD34+ baboon repopulating cells Blood, November 1, 2003; 102(9): 3117 - 3119. [Abstract] [Full Text] [PDF] J. Storek, T. Gillespy III, H. Lu, A. Joseph, M. A. Dawson, M. Gough, J. Morris, R. C. Hackman, P. A. Horn, G. E. Sale, et al. Interleukin-7 improves CD4 T-cell reconstitution after autologous CD34 cell transplantation in monkeys Blood, May 15, 2003; 101(10): 4209 - 4218. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020