SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on October 10, 2002; DOI 10.1182/blood-2002-05-1488. This Article Full Text (PDF) All Versions of this Article: 2002-05-1488v1 101/5/1898    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Piliponsky, A. M Articles by Levi-Schaffer, F. Search for Related Content PubMed PubMed Citation Articles by Piliponsky, A. M Articles by Levi-Schaffer, F. Social Bookmarking                   What's this? Submitted May 21, 2002 Accepted October 2, 2002 Non-IgE-dependent activation of human lung and cord blood-derived mast cells is induced by eosinophil major basic protein and modulated by the membrane form of stem cell factor Adrian M Piliponsky, Gerald J Gleich, Arnon Nagler, Ilan Bar, and Francesca Levi-Schaffer* Department of Pharmacology, School of Pharmacy, Faculty of Medicine, The Hebrew University of Jerusalem, Jerusalem, Israel Departments of Immunology and Medicine, Mayo Clinic and Foundation, Rochester, MN, USA Department of Bone Marrow Transplantation, The Chaim Sheba Medical Center, Tel-Hashomer, Israel Department of Thoracic Surgery, Assaf Harofeh Medical Center, Zerifin, Israel * Corresponding author; email: fls{at}cc.huji.ac.il. The allergic reaction begins with the antigen-induced aggregation of occupied high affinity IgE receptors expressed on mast cell surface, their activation, and the release of pro-inflammatory mediators that cause the "early phase" of this process. In addition, mast cell activation induces the onset of a "late phase" reaction characterized by the tissue infiltration of inflammatory cells, mainly eosinophils. We have hypothesized that during the late phase mast cells interact with and are activated by eosinophils. Here we report that highly purified human lung mast cells became responsive to eosinophil major basic protein (MBP) when in co-culture with human lung fibroblasts. In addition, cord blood derived-mast cells maintained in co-culture with 3T3 fibroblasts released more histamine and prostaglandin (PG)-D2 in comparison to cells maintained in suspension. The fibroblast-derived membrane form of stem cell factor (SCF) was found to be involved in the mast cell increased responsiveness to MBP. In fact, cord blood-derived mast cells cocultured with 3T3 in the presence of antisense for SCF or co-cultured with fibroblasts that do not express the membrane form of SCF were inhibited in their histamine releasing activity towards MBP. In addition, this form of SCF induced the expression of a pertussis toxin (Ptx)-sensitive Gi protein, Gi3, that interacts with MBP to trigger mast cell non-IgE dependent activation in a manner similar to other cationic compounds such as compound 48/80. Mast cell responsiveness to eosinophil mediators is potentially novel evidence for an alternative pathway of allergen-independent activation able to contribute to the perpetuation of allergy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. A. Plager, M. D. P. Davis, A. G. Andrews, M. J. Coenen, T. J. George, G. J. Gleich, and K. M. Leiferman Eosinophil Ribonucleases and Their Cutaneous Lesion-Forming Activity J. Immunol., September 15, 2009; 183(6): 4013 - 4020. [Abstract] [Full Text] [PDF] A.H. Nissim Ben Efraim and F. Levi-Schaffer Review: Tissue remodeling and angiogenesis in asthma: the role of the eosinophil Therapeutic Advances in Respiratory Disease, June 1, 2008; 2(3): 163 - 171. [Abstract] [PDF] I. Bachelet, A. Munitz, B. Berent-Maoz, D. Mankuta, and F. Levi-Schaffer Suppression of Normal and Malignant Kit Signaling by a Bispecific Antibody Linking Kit with CD300a J. Immunol., May 1, 2008; 180(9): 6064 - 6069. [Abstract] [Full Text] [PDF] B. Berent-Maoz, A. M. Piliponsky, I. Daigle, H.-U. Simon, and F. Levi-Schaffer Human Mast Cells Undergo TRAIL-Induced Apoptosis J. Immunol., February 15, 2006; 176(4): 2272 - 2278. [Abstract] [Full Text] [PDF] I. Bachelet, A. Munitz, A. Moretta, L. Moretta, and F. Levi-Schaffer The Inhibitory Receptor IRp60 (CD300a) Is Expressed and Functional on Human Mast Cells J. Immunol., December 15, 2005; 175(12): 7989 - 7995. [Abstract] [Full Text] [PDF] M. J. Hessner, X. Wang, L. Meyer, R. Geoffrey, S. Jia, J. Fuller, A. Lernmark, and S. Ghosh Involvement of Eotaxin, Eosinophils, and Pancreatic Predisposition in Development of Type 1 Diabetes Mellitus in the BioBreeding Rat J. Immunol., December 1, 2004; 173(11): 6993 - 7002. [Abstract] [Full Text] [PDF] P. G. Gibson Cough Is an Airway Itch? Am. J. Respir. Crit. Care Med., January 1, 2004; 169(1): 1 - 2. [Full Text] [PDF] G. Sellge, A. Lorentz, T. Gebhardt, F. Levi-Schaffer, H. Bektas, M. P. Manns, D. Schuppan, and S. C. Bischoff Human Intestinal Fibroblasts Prevent Apoptosis in Human Intestinal Mast Cells by a Mechanism Independent of Stem Cell Factor, IL-3, IL-4, and Nerve Growth Factor J. Immunol., January 1, 2004; 172(1): 260 - 267. [Abstract] [Full Text] [PDF] A. D. Klion, P. Noel, C. Akin, M. A. Law, D. G. Gilliland, J. Cools, D. D. Metcalfe, and T. B. Nutman Elevated serum tryptase levels identify a subset of patients with a myeloproliferative variant of idiopathic hypereosinophilic syndrome associated with tissue fibrosis, poor prognosis, and imatinib responsiveness Blood, June 15, 2003; 101(12): 4660 - 4666. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020