|
|
Prepublished online as a Blood First Edition Paper on December 27, 2002; DOI 10.1182/blood-2002-05-1501.
Submitted May 22, 2002
Accepted December 17, 2002
A novel myeloid-like NK cell progenitor in human umbilical cord blood
Sonia A Perez*, Panagiota A Sotiropoulou, Dimitra G Gkika, Louisa G Mahaira, Dimitrios K Niarchos, Angelos D Gritzapis, Yiannis G Kavalakis, Aris I Antsaklis, Constantin N Baxevanis, and Michael Papamichail
Cancer Immunology Immunotherapy Center, Saint Savas Hospital, Athens, Greece
1st Obstetrics and Gynecology University Clinic, Alexandras Maternity Hospital, Athens, Greece
* Corresponding author; email: cacenter{at}otenet.gr.
NK cell differentiation from pluripotent CD34+ human hematopoietic stem cells (HSC) or oligopotent lymphoid progenitors has already been reported. In the present study, long-term cultures of the CD56-/CD34- myeloid-like adherent cell fraction (ACF) from umbilical cord blood (UCB), characterized by the expression of CD14+ as well as other myeloid markers, were setup with flt3-ligand (FL) and IL-15. The UCB/ACF gradually expressed the CD56 marker, which reached fairly high levels (approximately 90% of the cells were CD56+) by day 15. FL plus IL-15-driven ACF/CD56+ cells progressively expressed a mature NK functional program lysing both NK- and LAK-sensitive tumor targets and producing high levels of IFN- , GM-CSF, TNF and IL-10 upon stimulation with IL-12 and IL-18. Similar results were obtained when highly purified CD14+ cells from UCB were cultured with FL and IL-15. In contrast, UCB/CD34+ cells cultured under the same conditions showed a delayed expression of CD56 and behaved functionally differently in that they exhibited NK- but not LAK- cytotoxicity and produced significantly less amounts of cytokines. Kinetic studies on the phenotype of UCB/ACF or UCB/CD14+ cells cultured in the presence of FL and IL-15 showed a rapid decrease in CD14 expression after day 5, which reached zero levels by day 20. Approximately 60% of the CD56+ derived from the UCB/ACF or the UCB/CD14+ cells co-expressed CD14 by day 5. Taken altogether, our data support the role of CD14+ myeloid-like cells within UCB as a novel progenitor for lymphoid NK cells.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
C. Vitale, F. Cottalasso, E. Montaldo, L. Moretta, and M. C. Mingari
Methylprednisolone induces preferential and rapid differentiation of CD34+ cord blood precursors toward NK cells
Int. Immunol.,
April 1, 2008;
20(4):
565 - 575.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Grzywacz, N. Kataria, M. Sikora, R. A. Oostendorp, E. A. Dzierzak, B. R. Blazar, J. S. Miller, and M. R. Verneris
Coordinated acquisition of inhibitory and activating receptors and functional properties by developing human natural killer cells
Blood,
December 1, 2006;
108(12):
3824 - 3833.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Perez, L. G. Mahaira, P. A. Sotiropoulou, A. D. Gritzapis, E. G. Iliopoulou, D. K. Niarchos, N. T. Cacoullos, Y. G. Kavalakis, A. I. Antsaklis, N. N. Sotiriadou, et al.
Effect of IL-21 on NK cells derived from different umbilical cord blood populations
Int. Immunol.,
January 1, 2006;
18(1):
49 - 58.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. A. Perez, L. G. Mahaira, F. J. Demirtzoglou, P. A. Sotiropoulou, P. Ioannidis, E. G. Iliopoulou, A. D. Gritzapis, N. N. Sotiriadou, C. N. Baxevanis, and M. Papamichail
A potential role for hydrocortisone in the positive regulation of IL-15-activated NK-cell proliferation and survival
Blood,
July 1, 2005;
106(1):
158 - 166.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Sconocchia, H. Fujiwara, K. Rezvani, K. Keyvanfar, F. El Ouriaghli, M. Grube, J. Melenhorst, N. Hensel, and A. J. Barrett
G-CSF-mobilized CD34+ cells cultured in interleukin-2 and stem cell factor generate a phenotypically novel monocyte
J. Leukoc. Biol.,
December 1, 2004;
76(6):
1214 - 1219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
