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Prepublished online as a Blood First Edition Paper on February 27, 2003; DOI 10.1182/blood-2002-05-1504. This Article Full Text (PDF) All Versions of this Article: 2002-05-1504v1 101/12/4870    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Odeberg, J. Articles by Soderberg-Naucler, C. Search for Related Content PubMed PubMed Citation Articles by Odeberg, J. Articles by Soderberg-Naucler, C. Social Bookmarking                   What's this? Submitted May 22, 2002 Accepted January 23, 2003 The human cytomegalovirus protein pp65 mediates accumulation of HLA-DR in lysosomes and destruction of the HLA-DR -chain Jenny Odeberg, Bodo Plachter, Lars Branden, and Cecilia Soderberg-Naucler* Departent of Molecular Medicine, Karolinska Institutet, Stockholm, Sweden Institute for Virology, University of Mainz, Mainz, Germany Clinical Research Center (CRC), Karolinska Institutet, Huddinge, Sweden * Corresponding author; email: cecilia.soderberg.naucler{at}cmm.ki.se. Human cytomegalovirus (HCMV) has developed multiple strategies to escape immune recognition. Here, we demonstrate that HCMV downregulates HLA-DR expression in infected IFN- stimulated fibroblasts at 1 day post infection. Decreased HLA-DR expression was not observed in cells infected with an HCMV strain lacking the pp65 gene (RVAD65), but in cells transfected with the pp65 gene. HLA-DR expression accumulated in vacuoles near the nucleus in HCMV infected, but not in uninfected or RVAD65 infected cells. In addition, the HLA-DR -chain, but not the [[beta]-chain or HLA-DM was degraded in HCMV infected, but not in RVAD65 infected cells. Thus, the HCMV protein pp65 mediates decreased expression of HLA-DR, by mediating an accumulation of HLA class II molecules in lysosmes that results in degradation of the HLA-DR-chain. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Chevillotte, S. Landwehr, L. Linta, G. Frascaroli, A. Luske, C. Buser, T. Mertens, and J. von Einem Major Tegument Protein pp65 of Human Cytomegalovirus Is Required for the Incorporation of pUL69 and pUL97 into the Virus Particle and for Viral Growth in Macrophages J. Virol., March 15, 2009; 83(6): 2480 - 2490. [Abstract] [Full Text] [PDF] M. Baron and J.-L. 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