SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on September 5, 2002; DOI 10.1182/blood-2002-05-1553. This Article Full Text (PDF) All Versions of this Article: 2002-05-1553v1 101/2/541    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Raslova, H. Articles by Vainchenker, W. Search for Related Content PubMed PubMed Citation Articles by Raslova, H. Articles by Vainchenker, W. Social Bookmarking                   What's this? Submitted May 28, 2002 Accepted August 21, 2002 Megakaryocyte polyploidization is associated with a functional gene amplification Hana Raslova, Lydia Roy, Claire Vourc'h, Jean P LeCouedic, Olivier Brison, Didier Metivier, Jean Feunteun, Guido Kroemer, Najet Debili, and William Vainchenker* Institut Gustave Roussy PR-1, INSERM Unite 362, Villejuif Cedex, France Institut Albert Bonniot, INSERM Unite 309, La Tronche, France Institut Gustave Roussy, CNRS UMR.1599, Villejuif Cedex, France * Corresponding author; email: verpre{at}igr.fr. It is believed that polyploidy induces an orchestrated increase in gene expression. To know whether all alleles remain functional during megakaryocyte polyploidization, we used a well-established FISH technique which allows one to simultaneously detect pre-mRNAs and assess ploidy level in a single cell. All alleles of GPIIb, GPIIIa, vWF, ß-actin, hsp70, c-mpl, Fli-1 and FOG-1 genes are transcriptionally active in megakaryocytes from 4N to 32N. All X chromosomes in male cells but only half of them are transcriptionally active in female megakaryocytes, as revealed by the transcriptional activity of the GATA-1 gene. Nuclear untranslated XIST RNA accumulates on the inactivated X chromosomes, indicating that they are subjected to a normal inactivation process. Altogether, our results demonstrate that megakaryocyte polyploidization results in a functional gene amplification whose likely function is an increase in protein synthesis in parallel to cell enlargement. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. Raslova, A. Kauffmann, D. Sekkai, H. Ripoche, F. Larbret, T. Robert, D. T. Le Roux, G. Kroemer, N. Debili, P. Dessen, et al. Interrelation between polyploidization and megakaryocyte differentiation: a gene profiling approach Blood, April 15, 2007; 109(8): 3225 - 3234. [Abstract] [Full Text] [PDF] R. N. Carter, G. Tolhurst, G. Walmsley, M. Vizuete-Forster, N. Miller, and M. P. Mahaut-Smith Molecular and electrophysiological characterization of transient receptor potential ion channels in the primary murine megakaryocyte J. Physiol., October 1, 2006; 576(1): 151 - 162. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020