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Prepublished online as a Blood First Edition Paper on December 12, 2002; DOI 10.1182/blood-2002-06-1830. This Article Full Text (PDF) All Versions of this Article: 2002-06-1830v1 101/8/2999    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Devine, S. M Articles by Hoffman, R. Search for Related Content PubMed PubMed Citation Articles by Devine, S. M Articles by Hoffman, R. Social Bookmarking                   What's this? Submitted June 20, 2002 Accepted November 22, 2002 Mesenchymal stem cells distribute to a wide range of tissues following systemic infusion into non-human primates Steven M Devine*, Carrington Cobbs, Matt Jennings, Amelia Bartholomew, and Ronald Hoffman Hematology/Oncology, University of Illinois at Chicago, Chicago, IL, USA Osiris Therapeutics, Inc., Baltimore, MD, USA Surgery, University of Illinois at Chicago, Chicago, IL, USA * Corresponding author; email: sdevine{at}im.wustl.edu. Ex vivo expanded mesenchymal stem cells (MSC) were transduced with a green fluorescent protein (GFP) retroviral construct and subsequently infused into three adult baboons following lethal total body irradiation and hematopoietic support (2) or without any prior conditioning (1). In order to study the long term fate of these MSC, necropsies were performed between 9 and 21 months following MSC infusion and an average of 16 distinct tissues were recovered from each recipient and evaluated for the presence of the GFP transgene in purified genomic DNA by sensitive real time PCR. Two baboons received autologous and one allogeneic GFP-transduced MSC. Both allogeneic and autologous MSC appeared to distribute in a similar manner. Gastrointestinal tissues harbored high concentrations of transgene per microgram of DNA. Additional tissues including kidney, lung, liver, thymus, and skin were also found to contain relatively high amounts of DNA equivalents. Estimated levels of engraftment in these tissues ranged from 0.1 to 2.7%. The non-conditioned recipient appeared to have less abundant engraftment. These data suggest that MSC initially distribute broadly following systemic infusion and later may participate in ongoing cellular turnover and replacement in a wide variety of tissues. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H Aurich, M Sgodda, P Kaltwasser, M Vetter, A Weise, T Liehr, M Brulport, J G Hengstler, M M Dollinger, W E Fleig, et al. Hepatocyte differentiation of mesenchymal stem cells from human adipose tissue in vitro promotes hepatic integration in vivo Gut, April 1, 2009; 58(4): 570 - 581. [Abstract] [Full Text] [PDF] F. Wang, J. E. Dennis, A. Awadallah, L. A. Solchaga, J. Molter, Y. Kuang, N. Salem, Y. Lin, H. Tian, J. A. Kolthammer, et al. Transcriptional profiling of human mesenchymal stem cells transduced with reporter genes for imaging Physiol Genomics, March 3, 2009; 37(1): 23 - 34. [Abstract] [Full Text] [PDF] P. A. Walker, S. K. Shah, M. T. Harting, and C. S. 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