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Prepublished online as a Blood First Edition Paper on April 3, 2003; DOI 10.1182/blood-2002-06-1848. This Article Full Text (PDF) All Versions of this Article: 2002-06-1848v1 102/3/858    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Loo, P. F Articles by Hendriks, R. W Search for Related Content PubMed PubMed Citation Articles by van Loo, P. F Articles by Hendriks, R. W Social Bookmarking                   What's this? Submitted June 21, 2002 Accepted February 25, 2003 Impaired hematopoiesis in mice lacking the transcription factor Sp3 Pieter F van Loo, Peter Bouwman, Kam-Wing Ling, Sabine Middendorp, Guntram Suske, Frank Grosveld, Elaine Dzierzak, Sjaak Philipsen*, and Rudolf W Hendriks Department of Cell Biology, Erasmus MC, Rotterdam, The Netherlands Department of Immunology, Erasmus MC, Rotterdam, The Netherlands Institut fuer Molekularbiologie und Tumorforschung, Philipps-Universitaet Marburg, Marburg, Germany * Corresponding author; email: philipsen{at}ch1.fgg.eur.nl. As the zinc-finger transcription factor Sp3 has been implicated in the regulation of many hematopietic-specific genes, we analyzed the role of Sp3 in hematopoiesis. At embryonic day 18.5 (E18.5), Sp3-/- mice exhibit a partial arrest of T cell development in the thymus and B cell numbers are reduced in liver and spleen. However, pre-B cell proliferation and differentiation into IgM+ B cells in vitro are not affected. At E14.5 and E16.5 Sp3-/- mice exhibit a significant delay in the appearance of definitive erythrocytes in the blood, paralleled by a defect in the progression of differentiation of definitive erythroid cells in vitro. Perinatal death of the null mutants precludes the analysis of adult hematopoiesis in Sp3-/- mice. We therefore investigated the ability of E12.5 Sp3-/- liver cells to contribute to the hematopoietic compartment in an in vivo transplantation assay. Sp3-/- cells were able to repopulate the B and T lymphoid compartment, albeit with reduced efficiency. In contrast, Sp3-/- cells showed no significant engraftment in the erythroid and myeloid lineages. Thus, the absence of Sp3 results in cell-autonomous hematopoietic defects, affecting in particular the erythroid and myeloid cell lineages. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Felli, F. Pedini, P. Romania, M. Biffoni, O. Morsilli, G. Castelli, S. Santoro, S. Chicarella, A. Sorrentino, C. Peschle, et al. MicroRNA 223-dependent expression of LMO2 regulates normal erythropoiesis Haematologica, April 1, 2009; 94(4): 479 - 486. [Abstract] [Full Text] [PDF] A. Montel-Hagen, L. Blanc, M. Boyer-Clavel, C. Jacquet, M. Vidal, M. Sitbon, and N. Taylor The Glut1 and Glut4 glucose transporters are differentially expressed during perinatal and postnatal erythropoiesis Blood, December 1, 2008; 112(12): 4729 - 4738. [Abstract] [Full Text] [PDF] P. F. van Loo, E. A. F. Mahtab, L. J. Wisse, J. Hou, F. Grosveld, G. Suske, S. Philipsen, and A. C. Gittenberger-de Groot Transcription Factor Sp3 Knockout Mice Display Serious Cardiac Malformations Mol. Cell. Biol., December 15, 2007; 27(24): 8571 - 8582. [Abstract] [Full Text] [PDF] A. P. W. Funnell, C. A. Maloney, L. J. Thompson, J. Keys, M. Tallack, A. C. Perkins, and M. Crossley Erythroid Kruppel-Like Factor Directly Activates the Basic Kruppel-Like Factor Gene in Erythroid Cells Mol. Cell. Biol., April 1, 2007; 27(7): 2777 - 2790. [Abstract] [Full Text] [PDF] N. J. Dempsey, L. S. Ojalvo, D. W. Wu, and J. A. Little Induction of an embryonic globin gene promoter by short-chain fatty acids Blood, December 1, 2003; 102(12): 4214 - 4222. [Abstract] [Full Text] [PDF]

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