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Prepublished online as a Blood First Edition Paper on September 26, 2002; DOI 10.1182/blood-2002-06-1861. This Article Full Text (PDF) All Versions of this Article: 2002-06-1861v1 101/3/903    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Heck, S. Articles by Graf, T. Search for Related Content PubMed PubMed Citation Articles by Heck, S. Articles by Graf, T. Social Bookmarking                   What's this? Submitted June 24, 2002 Accepted August 16, 2002 Distinguishable live erythroid and myeloid cells in ß-globin ECFP x lysozyme EGFP mice Susanne Heck, Olga Ermakova, Hiromi Iwasaki, Koichi Akashi, Chiao-Wang Sun, Thomas M Ryan, Tim Townes, and Thomas Graf* Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, Bronx, NY, USA Department of Cancer Immunology and AIDS, Dana Farber Cancer Center, Harvard Medical School, Boston, MA, USA Department of Biochemistry and Molecular Geneticslecular Genetics, University of Alabama, Birmingham, Birmingham, AL, USA * Corresponding author; email: graf{at}aecom.yu.edu. We have previously described a mouse line that contains green myelomonocytic cells due to the knock-in of EGFP into the lysozyme M gene [1]. We have now created a transgenic line with cyan fluorescent erythroid cells using a ß-globin LCR driving the ECFP gene. These mice exhibit cyan fluorescent cells specifically in the erythroid compartment and in megakaryocyte-erythroid progenitors (MEPs), allowing to track live definitive erythroid cells for the first time. Crossing the animals with lysozyme EGFP mice yielded a line in which live erythroid and myeloid cells can be readily distinguished by fluorescence microscopy and by FACS. The new mouse lines should become useful tools for the direct visualization of the branching between erythroid and myelomonocytic cells during in vitro differentiation of definitive multipotent progenitors. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: N. Suzuki, S. Imagawa, C. T. Noguchi, M. Yamamoto, and U. Klingmuller Do {beta}-globin, GATA-1,or EpoR regulatory domains specifically mark erythroid progenitors in transgenic reporter mice? Blood, November 1, 2004; 104(9): 2988 - 2989. [Full Text] [PDF]

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