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Prepublished online as a Blood First Edition Paper on January 9, 2003; DOI 10.1182/blood-2002-06-1866.
Submitted June 24, 2002
Accepted December 7, 2002
Gene expression profile in human leukocytes
Shin-ichi Hashimoto, Shigenori Nagai, Jun Sese, Takuji Suzuki, Aya Obata, Taku Sato, Nobuaki Toyoda, Hong-Yan Dong, Makoto Kurachi, Tomoyuki Nagahata, Ken-ichi Shizuno, Shinichi Morishita, and Kouji Matsushima*
Department of Molecular Preventive Medicine, University of Tokyo School of Medicine, Tokyo, Japan
Department of Complexity Science and Engineering, University of Tokyo Graduate School of Frontier Science, Tokyo, Japan
* Corresponding author; email: koujim{at}m.u-tokyo.ac.jp.
Leukocytes are classified to myelocytic and lymphocytic leukocytes, and each class of the leukocytes consists of several types of cells which have different phenotypes and different roles. In order to define the gene expression in these cells, we have performed serial analysis of gene expression (SAGE) using human leukocytes, and provided the gene data base for these cells not only of the resting stage but also of the activated stage. A total of 709,990 tags from 17 libraries were analyzed for the manifestation of gene expression profiles in various types of human leukocytes. The types of leukocytes analyzed were as follows; peripheral blood monocytes, colony-stimulating factor-induced macrophages, monocytes-derived immature dendritc cells, mature/activated dendritic cells, granulocytes, NK cells, resting B cells, activated B cells, naive T cells, CCR4- memory T cells(resting Th1 cells), CCR4+ memory T cells (resting Th2 cells), activated Th1 cells and activated Th2 cells. Among 38,961 distinct tags that appeared more than once in the combined total libraries, 27,323 tags were found to represent unique genes in certain type(s) of leukocytes. By P-chance and hierarchical clustering analyses we identified the genes that are selectively expressed in each type of leukocytes. Identification of the genes specifically expressed in different types of leukocytes provides not only novel molecular signature to define different subsets of resting and activated cells but also contributes to further understanding of the biological function of leukocytes in the host defense system.
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