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Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-06-1929. This Article Full Text (PDF) All Versions of this Article: 2002-06-1929v1 101/5/1891    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Malphettes, M. Articles by Autran, B. Search for Related Content PubMed PubMed Citation Articles by Malphettes, M. Articles by Autran, B. Social Bookmarking                   What's this? Submitted June 28, 2002 Accepted October 5, 2002 Evidence for naive T-cell repopulation despite thymus irradiation after autologous transplantation in adults with multiple myeloma: role of ex-vivo CD34+ selection and age Marion Malphettes, Guislaine Carcelain, Pierre Saint-Mezard, Veronique Leblond, Hester Korthals Altes, Jean-Pierre Marolleau, Patrice Debre, Jean-Claude Brouet, Jean-Paul Fermand, and Brigitte Autran* Laboratoire d'Immunologie Cellulaire et Tissulaire URA CNRS 625, Hopital de La Pitie-Salpetriere, Paris, France Service d'Immuno-Hematologie, Hopital Saint-Louis, Paris, France Service d'Hematologie, Hopital de La Pitie-Salpetriere, Paris, France * Corresponding author; email: brigitte.autran{at}psl.ap-hop-paris.fr. Immunodeficiency following autologous CD34+ purified peripheral blood stem cell (PBSC) transplantation could be related to T-cell depletion of the graft and/or impaired T-cell reconstitution due to thymus irradiation. Aiming to assess the role of irradiated thymus in T-cell repopulation, we studied 32 adults with multiple myeloma, randomly assigned to receive high dose therapy including total body irradiation (TBI) followed by autologous transplantation with either unselected or CD34+ selected PBSC. The median number of reinfused CD3+ cells was lower in the selected group (0.03 versus 14 x 106/kg; p=0.002). Lymphocyte subset counts were evaluated from Month 3 to 24 post-graft. Naive CD4+ T-cells were characterized both by phenotype and by T-cell receptor rearrangement excision circles (TRECs) quantification. The reconstitution of CD3+ and CD4+ T-cells was significantly delayed in the CD34+ selected group, but eventually led to similar counts than in the unselected group after Month 12. Mechanism of reconstitution differed, however, between both groups. Indeed, a marked increase in the naive CD62L+CD45RA+CD4+ subset was observed in the selected group, but not in the unselected group in which half of CD45RA+CD4+ T-cells appear to be CD62L-negative. Age was identified as an independent adverse factor for CD4+ and CD62L+ CD45RA+CD4+ T-cell reconstitution. Our results provide evidence that infusing T-cell depleted PBSCs after TBI in adults delays T-cell reconstitution but accelerates thymic regeneration. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J.-H. Bourhis, Y. Bouko, S. Koscielny, M. Bakkus, H. Greinix, G. Derigs, G. Salles, W. Feremans, J. Apperley, D. Samson, et al. Relapse risk after autologous transplantation in patients with newly diagnosed myeloma is not related with infused tumor cell load and the outcome is not improved by CD34+ cell selection: long term follow-up of an EBMT phase III randomized study Haematologica, August 1, 2007; 92(8): 1083 - 1090. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020