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Prepublished online as a Blood First Edition Paper on October 31, 2002; DOI 10.1182/blood-2002-07-1995. This Article Full Text (PDF) All Versions of this Article: 2002-07-1995v1 101/8/2905    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yahata, T. Articles by Hotta, T. Search for Related Content PubMed PubMed Citation Articles by Yahata, T. Articles by Hotta, T. Related Collections Related Letter in Blood Online Social Bookmarking                   What's this? Submitted July 8, 2002 Accepted October 24, 2002 A highly sensitive strategy for scid-repopulating cell assay by direct injection of primitive human hematopoietic cells into NOD/SCID mice bone marrow Takashi Yahata, Kiyoshi Ando*, Tadayuki Sato, Hiroko Miyatake, Yoshihiko Nakamura, Yukari Muguruma, Shunichi Kato, and Tomomitsu Hotta Division of Hematopoiesis, Reserch center for Regenerative Medicine, Tokai University, School of Medicine, Kanagawa, Japan Department of Hematology, Tokai University, School of Medicine, Kanagawa, Japan Institute of Medical Sciences, Tokai University, School of Medicine, Kanagawa, none * Corresponding author; email: andok{at}keyaki.cc.u-tokai.ac.jp. To measure the ability of human hematopoietic stem cells (HSCs), SCID-repopulating cell (SRC) assay has been widely used. Conventionally, human HSCs are transplanted into NOD/SCID mouse via a tail vein. However, those cells must go through various obstacles until they reach the mouse marrow environment, which could explain the generally low homing efficiency in this system. Thus, the capability of HSCs may not be studied accurately by this intravenous (iv) transplantation method. In our attempt to reveal actual SRC potential, (i.e.) self-renewal and multi-lineage differentiation in recipient bone marrow, we introduced cells into mouse marrow directly (intra-bone marrow [iBM]) to minimize the effect of factors which may interfere the homing of HSCs and compared the results obtained by iv and iBM methods. When cord blood CD34+CD38- cells were transplanted in NOD/SCID mice by iBM, a 15-fold higher frequency of SRC, 1 in 44 CD34+CD38- cells, was achieved compared with 1 in 660 by iv. Furthermore, the iBM transplant showed high levels of engraftment in the secondary transplantation. Pretreatment of CD34+ cells with antibodies which block either VLA-4 or VLA-5 reduced engraftment partially while blockage of both molecules resulted in complete inhibition of engraftment, which suggests that VLA-4 and VLA-5 are involved in different processes in engraftment or have complementary roles. Our results indicate that the iBM injection strategy is more sensitive and direct way to measure the capability of human SRCs and is useful to investigate the interaction of HSCs and marrow environment in vivo. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? Related Letter in Blood Online: Direct vascular delivery of primitive hematopoietic cells to bone marrow improves localization but not engraftment Karissa T. Chabner, Gregor B. Adams, Jianhua Qiu, Michael Moskowitz, Emily S. Marsters, George P. Topulos, and David T. Scadden Blood 2004 103: 4685-4686. [Full Text] [PDF] This article has been cited by other articles: Z. Zeng, Y. Xi Shi, I. J. 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