SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on August 22, 2002; DOI 10.1182/blood-2002-07-2064. This Article Full Text (PDF) All Versions of this Article: 2002-07-2064v1 101/2/752    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wagner, F. F Articles by Flegel, W. A Search for Related Content PubMed PubMed Citation Articles by Wagner, F. F Articles by Flegel, W. A Social Bookmarking                   What's this? Submitted July 10, 2002 Accepted August 8, 2002 The Scianna antigens including Rd are expressed by ERMAP Franz F Wagner, Joyce Poole, and Willy A Flegel* Transfusion Medicine, University of Ulm, Ulm, Germany; Institute Ulm, Red Cross Blood Service, Ulm, Germany International Blood Group Reference Laboratory, Bristol, United Kingdom * Corresponding author; email: waf{at}ucsd.edu. The Scianna blood group encompasses the high frequency antigens Sc1 and Sc3 and the low frequency antigen Sc2. Another low frequency antigen Rd (Radin) was suggested to belong to the Scianna blood group. The molecular basis of the Scianna blood group was unknown. The erythrocyte membrane-associated protein (ERMAP) shared the genomic location, protein product size, and localization to the red blood cell (RBC) membrane surface with Scianna. The ERMAP gene was sequenced in probands with known Scianna and Radin phenotypes. In a Sc:-1,-2 proband, only an ERMAP allele with a two base pair deletion in exon 3 causing a frameshift could be detected. A Sc:-1,2 proband was homozygous for the ERMAP (G57R) allele. An Rd+ proband was heterozygous for the ERMAP (P60A) allele. PCR-SSP systems were developed to detect the Sc2 and Rd alleles of the ERMAP gene. The two alleles occurred with about 1% and <1% frequency in the population, which was compatible with the frequency of the Sc2 and Rd antigens known in Whites. Two Sc2+ and one Rd+ samples that were found by genotyping were confirmed by serology. The antigens of the Scianna blood group include Rd and are expressed by the human ERMAP protein. Sc2 is caused by an ERMAP (G57R) allele, Rd by an ERMAP (P60A) allele. Scianna is the last of the previously characterized protein based blood group systems whose molecular basis was discerned. Hence, the phenotype prediction by genotyping became possible for all human blood group systems encoded by proteins. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: D. Hodge, E. Coghill, J. Keys, T. Maguire, B. Hartmann, A. McDowall, M. Weiss, S. Grimmond, and A. Perkins A global role for EKLF in definitive and primitive erythropoiesis Blood, April 15, 2006; 107(8): 3359 - 3370. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020