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Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-07-2146. This Article Full Text (PDF) All Versions of this Article: 2002-07-2146v1 101/7/2551    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by De Geest, B. R Articles by Collen, D. Search for Related Content PubMed PubMed Citation Articles by De Geest, B. R Articles by Collen, D. Social Bookmarking                   What's this? Submitted July 18, 2002 Accepted November 7, 2002 Humoral immune response in mice against a circulating antigen induced by adenoviral transfer is strictly dependent on expression in antigen-presenting cells Bart R De Geest*, Sophie A Van Linthout, and Desire Collen Department for Molecular and Cardiovascular Research, Center for Molecular and Vascular Biology, Leuven, Belgium * Corresponding author; email: Bart.Degeest{at}med.kuleuven.ac.be. Adenoviral transfer of human apo A-I in Balb/c mice induces a strong humoral immune response against the transgene product when expression is driven from the ubiquitously active CMV promoter but no immune response when driven by the hepatocyte-specific 256 bp apo A-I promoter. Here the hypothesis, that the humoral immune response against the circulating transgene product correlates with its expression in antigen presenting cells, was tested. No humoral immune response was observed after adenoviral transfer of vectors with human apo A-I expression driven by the hepatocyte-specific apo C-II or 1.5 kb human 1-antitrypsin promoter, but antibodies were induced after transfer with vectors driven by the ubiquitously active U1b promoter and the murine MHCII E promoter. A strict correlation was observed between antigen expression in the spleen and the occurrence of an immune response. Co-injection of the 1.5 kb human 1-antitrypsin and the murine MHCII E promoter driven vectors resulted in a very short-lived humoral immune response against human apo A-I, suggesting that the time-course of human apo A-I expression is a critical determinant of the development of tolerance for human apo A-I. High titers of antibodies against human apo A-I after subcutaneous gene transfer with the MHCII E promoter driven vector underscore the potential of this promoter for vaccination purposes. In conclusion, humoral immune response in mice against a circulating antigen induced by adenoviral transfer is strictly dependent on expression in antigen-presenting cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. D. Brown, A. Cantore, A. Annoni, L. S. Sergi, A. Lombardo, P. Della Valle, A. D'Angelo, and L. Naldini A microRNA-regulated lentiviral vector mediates stable correction of hemophilia B mice Blood, December 15, 2007; 110(13): 4144 - 4152. [Abstract] [Full Text] [PDF] A. Annoni, M. Battaglia, A. Follenzi, A. Lombardo, L. Sergi-Sergi, L. Naldini, and M.-G. Roncarolo The immune response to lentiviral-delivered transgene is modulated in vivo by transgene-expressing antigen-presenting cells but not by CD4+CD25+ regulatory T cells Blood, September 15, 2007; 110(6): 1788 - 1796. [Abstract] [Full Text] [PDF] O. Cao, E. Dobrzynski, L. Wang, S. Nayak, B. Mingle, C. Terhorst, and R. W. 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