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Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-07-2151. This Article Full Text (PDF) All Versions of this Article: 2002-07-2151v1 101/6/2318    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ogawa, H. Articles by Galili, U. Search for Related Content PubMed PubMed Citation Articles by Ogawa, H. Articles by Galili, U. Social Bookmarking                   What's this? Submitted July 18, 2002 Accepted October 21, 2002 Tolerance induction to a mammalian blood group like carbohydrate antigen by syngeneic lymphocytes expressing the antigen Haruko Ogawa, Deng-Ping Yin, Jikun Shen, and Uri Galili* Department of Cardivascular-Thoracic Surgery, Rush University, Chicago, IL, USA Department of Surgery, Rush University, Chicago, IL, USA Department of Cardivascular-Thoracic Surgery, Rush University, Chicago, IL, USA; Department of Immunology and Microbiology, Rush University, Chicago, IL, USA * Corresponding author; email: uri_galili{at}rush.edu. Tolerance induction to transplantation associated carbohydrate antigens, such as blood group A or B and the -gal epitope (Gal1-3Galß1-4GlcNAc-R), is of clinical significance. This study demonstrates tolerance induction to the -gal epitope in the experimental animal model of 1,3galactosyltransferanse knockout mice (KO mice lacking a-gal epitopes), by administering syngeneic lymphocytes expressing -gal epitopes. Repeated immunization of control KO mice with pig kidney membranes (PKM), expressing many a-gal epitopes, induces an extensive anti-Gal antibody response against this epitope. In contrast, KO mice that received as few as 2x106 wild type (WT) lymphocytes, were tolerized and failed to produce anti-Gal following PKM immunizations. Accordingly, control mice producing anti-Gal, rapidly rejected transplanted WT hearts, whereas tolerized mice did not reject WT hearts. These findings suggest that autologous blood lymphocytes processed to express a carbohydrate antigen may induce a similar tolerance to such antigen upon administration into humans. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: U. Abdel-Motal, S. Wang, S. Lu, K. Wigglesworth, and U. Galili Increased Immunogenicity of Human Immunodeficiency Virus gp120 Engineered To Express Gal{alpha}1-3Gal{beta}1-4GlcNAc-R Epitopes J. Virol., July 15, 2006; 80(14): 6943 - 6951. [Abstract] [Full Text] [PDF] N. Mitsuhashi, J. Fischer-Lougheed, I. Shulkin, A. Kleihauer, D. B. Kohn, K. I. Weinberg, V. A. Starnes, and M. Kearns-Jonker Tolerance induction by lentiviral gene therapy with a nonmyeloablative regimen Blood, March 15, 2006; 107(6): 2286 - 2293. [Abstract] [Full Text] [PDF] M. M. Mohiuddin, H. Ogawa, D.-P. Yin, and U. Galili Tolerance induction to a mammalian blood group--like carbohydrate antigen by syngeneic lymphocytes expressing the antigen, II: tolerance induction on memory B cells Blood, July 1, 2003; 102(1): 229 - 236. [Abstract] [Full Text] [PDF]

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