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Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-07-2224. This Article Full Text (PDF) Supplemental Figure All Versions of this Article: 2002-07-2224v1 101/6/2167    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Verhoeyen, E. Articles by Cosset, F.-L. Search for Related Content PubMed PubMed Citation Articles by Verhoeyen, E. Articles by Cosset, F.-L. Social Bookmarking                   What's this? Submitted July 24, 2002 Accepted October 22, 2002 IL-7 surface-engineered lentiviral vectors promote survival and efficient gene transfer in resting primary T lymphocytes Els Verhoeyen, Valerie Dardalhon, Odile Ducrey-Rundquist, Didier Trono, Naomi Taylor, and Francois-Loic Cosset* U412, ENS of Lyon, Lyon, France UMR5535, IGMM, Montpellier, France University of Geneva, Faculty of Medicine, Geneva, Switzerland * Corresponding author; email: flcosset{at}ens-lyon.fr. Important gene therapy target cells such as resting human T cells are refractory to transduction with lentiviral vectors. Completion of reverse transcription, nuclear import, and subsequent integration of the lentiviral genome only occur in these cells if they have been activated. In T cell based gene therapy trials performed to date, cells have been activated via their cognate antigen receptor. To couple activation with gene transfer, we previously generated lentiviral vectors displaying an anti-CD3 scFv fragment which allowed up to 48% transduction of freshly isolated T cells. However, transduction of highly purified resting T cells with these anti-CD3-displaying lentiviral vectors was inefficient and shifted the T cells from the naive to the memory phenotype. Here, we describe IL-7-displaying HIV-1-derived vectors. Like with recombinant IL-7, these modified particles could promote the survival of primary T cells placed in culture without inducing a naive to memory phenotypic switch. Furthermore, a single exposure to the IL-7-displaying vectors resulted in efficient gene transfer in both resting memory adult T cells and naive cord blood T cells. With adult naive T cells, pre-activation with recombinant IL-7 was necessary for efficient gene transfer. Altogether, these results suggest that IL-7-displaying vectors could constitute interesting tools for T-cell-targeted gene therapy. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. Frecha, C. Costa, C. Levy, D. Negre, S. J. Russell, A. Maisner, G. Salles, K.-W. Peng, F.-L. Cosset, and E. Verhoeyen Efficient and stable transduction of resting B lymphocytes and primary chronic lymphocyte leukemia cells using measles virus gp displaying lentiviral vectors Blood, October 8, 2009; 114(15): 3173 - 3180. [Abstract] [Full Text] [PDF] L. M. Agosto, J. J. Yu, M. K. Liszewski, C. Baytop, N. Korokhov, L. M. Humeau, and U. O'Doherty The CXCR4-Tropic Human Immunodeficiency Virus Envelope Promotes More-Efficient Gene Delivery to Resting CD4+ T Cells than the Vesicular Stomatitis Virus Glycoprotein G Envelope J. Virol., August 15, 2009; 83(16): 8153 - 8162. [Abstract] [Full Text] [PDF] E. Verhoeyen and F.-L. 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