SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-07-2244. This Article Full Text (PDF) All Versions of this Article: 2002-07-2244v1 101/9/3424    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Sanz, E. Articles by de la Hera, A. Search for Related Content PubMed PubMed Citation Articles by Sanz, E. Articles by de la Hera, A. Social Bookmarking                   What's this? Submitted July 24, 2002 Accepted October 29, 2002 Human cord blood CD34+Pax-5+ B-cell progenitors: single-cell analyses of their gene expression profiles Eva Sanz, Melchor Alvarez-Mon, Carlos Martinez-A., and Antonio de la Hera* Department of Medicine, Laboratory of Immune System Diseases and Oncology, CSIC Associated Unit, Alcala University, Madrid, Spain Immune System Disease and Oncology, Principe de Asturias University Hospital, Madrid, Spain Immunology and Oncology, Centro Nacional de Biotecnologia, Madrid, Spain * Corresponding author; email: adelahera{at}cib.csic.es. Circulating CD34+ cells are used in reparative medicine as a stem cell source, but they contain cells already committed to different lineages. Many think that B cell progenitors (BCP) are confined to bone marrow (BM) niches until they differentiate into B cells, and do not circulate in blood. The prevailing convention is that BCP transit a CD34+CD19--10+ early-B -> CD34+CD19+CD10+ B-cell progenitor (pro-B) -> CD34-CD19+CD10+ B-cell precursor (pre-B) differentiation pathway within BM. However, there are populations of CD34+CD10+ and CD34+CD19+ cells circulating in adult peripheral blood and neonatal umbilical cord blood (CB), that are operationally taken as BCP based on their phenotype although they have not been submitted to a systematic characterization of their gene expression profiles. Here, conventional CD34+CD19+CD10+ and novel CD34+CD19+CD10- BCP populations are characterized in CB by single-cell sorting and multiplex analyses of gene expression patterns. Circulating BCP are Pax-5+ cells that span the early-B, pro-B and pre-B developmental stages, defined by the profiles of rearranged V-D-JH, CD79, VpreB, recombination activating gene (RAG) and terminal deoxynucleotidyl transferase (TdT) expression. Contrary to the expectation, the circulating CD34+CD19-CD10+ cells are essentially devoid of Pax-5+ BCP. Interestingly, the novel CD34+CD19+CD10- BCP appears to be the normal counterpart of circulating pre-leukemic BCP that suffer chromosomal translocations in utero months or years before their promotion into infant acute lymphoblastic B-cell leukemia after secondary postnatal mutations. The results underline the power of single-cell analyses to characterize the gene expression profiles in minor population of rare cells, which has broad implications in biomedicine. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. E. Hystad, J. H. Myklebust, T. H. Bo, E. A. Sivertsen, E. Rian, L. Forfang, E. Munthe, A. Rosenwald, M. Chiorazzi, I. Jonassen, et al. Characterization of Early Stages of Human B Cell Development by Gene Expression Profiling J. Immunol., September 15, 2007; 179(6): 3662 - 3671. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020