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Prepublished online as a Blood First Edition Paper on November 27, 2002; DOI 10.1182/blood-2002-07-2265. This Article Full Text (PDF) All Versions of this Article: 2002-07-2265v1 101/7/2601    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Ito, A. Articles by Kitamura, Y. Search for Related Content PubMed PubMed Citation Articles by Ito, A. Articles by Kitamura, Y. Social Bookmarking                   What's this? Submitted July 29, 2002 Accepted November 19, 2002 SgIGSF: a new mast-cell adhesion molecule used for attachment to fibroblasts and transcriptionally regulated by MITF Akihiko Ito, Tomoko Jippo, Tomohiko Wakayama, Eiichi Morii, Yu-ichiro Koma, Hiroaki Onda, Hiroshi Nojima, Shoichi Iseki, and Yukihiko Kitamura* Department of Pathology, Osaka University Medical School/Graduate School of Frontier Bioscience, Suita, Osaka, Japan Department of Histology and Embryology, Graduate School of Medical Science, Kanazawa University, Kanazawa, Ishikawa, Japan Department of Molecular Genetics, Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan * Corresponding author; email: kitamura{at}patho.med.osaka-u.ac.jp. MITF is a basic-helix-loop-helix-leucine zipper-type transcription factor. The mutant mi and Miwh alleles encode MITFs with deletion and alteration of a single amino acid, respectively, while the tg is a null mutation. In coculture with NIH/3T3 fibroblasts, numbers of cultured mast cells (CMCs) derived from C57BL/6 (B6)-mi/mi, B6-Miwh/Miwh and B6-tg/tg mice that adhered to NIH/3T3 fibroblasts were one third that of B6-+/+ CMCs. We subtracted a cDNA library of B6-+/+ CMCs with messenger RNAs expressed by B6-mi/mi CMCs and found a clone encoding SgIGSF, a recently identified member of the immunoglobulin superfamily. Northern and Western blot analyses revealed that SgIGSF was expressed in B6-+/+ CMCs but not in CMCs derived from MITF mutants. Immunocytochemical analysis showed that SgIGSF localized to the cell-to-cell contact areas between B6-+/+ CMCs and NIH/3T3 fibroblasts. Transfection of B6-mi/mi and B6-tg/tg CMCs with SgIGSF cDNA normalized their adhesion to NIH/3T3 fibroblasts. NIH/3T3 fibroblasts did not express SgIGSF, indicating that SgIGSF acts as a heterophilic adhesion molecule. Transfection of B6-tg/tg CMCs with normal MITF cDNA elevated their SgIGSF expression to normal levels. 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