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Prepublished online as a Blood First Edition Paper on December 27, 2002; DOI 10.1182/blood-2002-07-2277. This Article Full Text (PDF) All Versions of this Article: 2002-07-2277v1 101/8/3085    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Nath, M. D Articles by Jones, K. S Search for Related Content PubMed PubMed Citation Articles by Nath, M. D Articles by Jones, K. S Social Bookmarking                   What's this? Submitted July 29, 2002 Accepted December 5, 2002 Regulation of the cell surface expression of an HTLV-I-binding protein in human T cells during immune activation Manisha D Nath, Francis W Ruscetti*, Cari Petrow-Sadowski, and Kathryn S Jones Basic Research Laboratory, National Cancer Institute at Frederick, Frederick, MD, USA Basic Research Program, SAIC-Frederick Inc., Frederick, MD, USA * Corresponding author; email: ruscettif{at}mail.ncifcrf.gov. Little is known about the requirements for human T cell leukemia virus type I (HTLV-I) entry, including the identity of the cellular receptor(s). Recently, we have generated an HTLV-I SU immunoadhesin, HTSU-IgG, which binds specifically to cell surface protein(s) critical for HTLV-I mediated entry in cell lines. Here, expression of the HTLV-I SU binding protein on primary cells of the immune system was examined. The immunoadhesin specifically bound to adult T cells, B cells, NK cells, and macrophages. Cell stimulation dramatically increased the amount of binding with the highest levels of binding on CD4+ and CD8+ T cells. Naive (CD45RAhigh, CD62Lhigh) CD4+ T cells derived from cord blood cells, in contrast to other primary cells and all cell lines examined, bind no detectable HTLV-I SU. However, following stimulation, the level of HTSU-IgG binding was rapidly induced (<6 hours), reaching the level of binding seen on adult CD4+ T cells by 72 hours. In contrast to HTLV-I virions, the soluble HTSU-IgG did not effect T cell activation or proliferation. When incubated with human PBMCs in a mixed leukocyte reaction, HTSU-IgG inhibited proliferation at less than 1 ng/ml. These results indicate that cell surface expression of the HTLV SU binding protein is up-regulated during in vitro activation, and suggest a role for the HTLV-I SU binding proteins in the immunobiology of CD4+ T cells. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? 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