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Prepublished online as a Blood First Edition Paper on January 2, 2003; DOI 10.1182/blood-2002-08-2424. This Article Full Text (PDF) All Versions of this Article: 2002-08-2424v1 101/9/3574    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Greeve, J. Articles by Parwaresch, R. Search for Related Content PubMed PubMed Citation Articles by Greeve, J. Articles by Parwaresch, R. Social Bookmarking                   What's this? Submitted August 9, 2002 Accepted December 23, 2002 Expression of activation-induced cytidine deaminase in human B-cell non-Hodgkin's lymphomas Jobst Greeve*, Antje Philipsen, Kristina Krause, Wolfram Klapper, Klaus Heidorn, Brian E Castle, Joe Janda, Kenneth B Marcu, and Reza Parwaresch Medizinische Klinik I, Universitaetsklinikum Hamburg-Eppendorf, Hamburg, Germany Klinik fuer Allgemeine Innere Medizin, University Hospital Bern, Bern, Switzerland Institut fuer Haematopathologie und Lymphknotenregister der Deutschen Gesellschaft fuer Pathologie, Christian-Albrecht-Universitaet Kiel, Kiel, Germany Biology Department, Boehringer Ingelheim Pharmaceuticals, Ridgefield, CT, USA Biochemistry and Cell Biology Department, SUNY at Stoney Brook, Stony Brook, NY, USA * Corresponding author; email: greeve{at}uke.uni-hamburg.de. Activation-induced cytidine deaminase (AID) induces somatic hypermutations (SHM), class switch recombination (CSR) and immunoglobulin gene conversion in B-lymphocytes. Here we report for the first time the expression of AID in normal human B lymphocytes and in B cell non-Hodgkin`s lymphomas (B-NHL). AID mRNA expression in man is restricted to the CD19+CD38+IgD- germinal center cells, namely the CD19+CD38+CD44- centroblasts. After in vitro stimulation of naive human B cells by CD40-L and IL-4, AID mRNA is strongly induced for only 48 h. In a survey of human B-NHL, AID was found to be constitutively expressed in follicular lymphomas and in diffuse large B-cell lymphomas, but to be absent in B precursor lymphoblastic leukemia, in mantle cell lymphoma and in plasma cell myeloma. In B cell chronic lymphatic leukemia, in immunocytoma and in extranodal marginal zone B cell lymphoma of MALT, AID mRNA was expressed only in some samples. In follicular lymphomas and diffuse large B cell lymphomas, the expression of AID mRNA was coincident with the presence of SHM in the variable region exons of the immunoglobulin heavy chain gene. In human B-NHL, the AID mRNA is spliced into four different variants, but does not contain point mutations. Thus, AID which is highly regulated during normal B cell development is constitutively expressed in human germinal center B-NHLs and also in subsets of non-germinal center B-NHLs. This constitutive expression of AID may promote illegitimate DNA recombinations and somatic mutations in B-NHLs. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: R. J. Bende, F. van Maldegem, and C. J.M. van Noesel Chronic inflammatory disease, lymphoid tissue neogenesis and extranodal marginal zone B-cell lymphomas Haematologica, August 1, 2009; 94(8): 1109 - 1123. [Abstract] [Full Text] [PDF] F. Colotta, P. Allavena, A. Sica, C. Garlanda, and A. Mantovani Cancer-related inflammation, the seventh hallmark of cancer: links to genetic instability Carcinogenesis, July 1, 2009; 30(7): 1073 - 1081. 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