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Prepublished online as a Blood First Edition Paper on May 15, 2003; DOI 10.1182/blood-2002-08-2426. This Article Full Text (PDF) All Versions of this Article: 2002-08-2426v1 102/5/1661    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wilson, H. L Articles by O'Neill, H. C Search for Related Content PubMed PubMed Citation Articles by Wilson, H. L Articles by O'Neill, H. C Social Bookmarking                   What's this? Submitted August 9, 2002 Accepted May 4, 2003 Identification of differentially expressed genes representing dendritic cell precursors and their progeny Heather L Wilson and Helen C O'Neill* School of Biochemistry and Molecular Biology, The Australian National University, Canberra, ACT, Australia * Corresponding author; email: Helen.ONeill{at}anu.edu.au. The development of dendritic cells (DC) from hematopoietic progenitors is not well understood. Using a spleen-derived long-term culture (LTC) system, it has been possible to continuously generate DC from progenitors maintained in culture. The non-adherent LTC-DC population is comprised of two major subsets. These are the small LTC-DC or DC precursors and their progeny, the large LTC-DC which phenotypically resemble immature DC. In this study, subtracted cDNA libraries were generated containing sequences differentially expressed in either small or large LTC-DC. Differential screening was then used on plated library clones to select genes expressed in either the small or large cell population. Real-time PCR has been used to verify the selection procedure for several genes of particular interest. Known genes isolated from subtracted libraries related to stages in DC development and supported previous findings regarding the function of small and large LTC-DC. Large LTC-DC expressed a number of immunologically important genes including CD86, CCR1, osteopontin and lysozyme. Small LTC-DC resembled progenitor DC expressing genes relating to the organisation of the cytoskeleton, regulation of antigen processing and a number of mitochondrial and ribosomal proteins. Novel transcripts were isolated from both small and large LTC-DC subtracted libraries which could encode novel proteins important in DC development. This study describes changes in gene expression related to the development of CD11c+CD11b+MHCIIloCD8- DC from precursors in a stroma-dependent culture system in the absence of exogenous cytokines. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Illario, M. L. Giardino-Torchia, U. Sankar, T. J. Ribar, M. Galgani, L. Vitiello, A. M. Masci, F. R. Bertani, E. Ciaglia, D. Astone, et al. Calmodulin-dependent kinase IV links Toll-like receptor 4 signaling with survival pathway of activated dendritic cells Blood, January 15, 2008; 111(2): 723 - 731. [Abstract] [Full Text] [PDF] S. J. A. M. Santegoets, A. J. Masterson, P. C. van der Sluis, S. M. Lougheed, D. M. Fluitsma, A. J. M. van den Eertwegh, H. M. Pinedo, R. J. Scheper, and T. D. de Gruijl A CD34+ human cell line model of myeloid dendritic cell differentiation: evidence for a CD14+CD11b+ Langerhans cell precursor J. Leukoc. Biol., December 1, 2006; 80(6): 1337 - 1344. [Abstract] [Full Text] [PDF] P. J. Cejas, L. M. Carlson, D. Kolonias, J. Zhang, I. Lindner, D. D. Billadeau, L. H. Boise, and K. P. Lee Regulation of RelB Expression during the Initiation of Dendritic Cell Differentiation Mol. Cell. Biol., September 1, 2005; 25(17): 7900 - 7916. [Abstract] [Full Text] [PDF] J. K. H. Tan and H. C. O'Neill Maturation requirements for dendritic cells in T cell stimulation leading to tolerance versus immunity J. Leukoc. Biol., August 1, 2005; 78(2): 319 - 324. [Abstract] [Full Text] [PDF] A. C. Renkl, J. Wussler, T. Ahrens, K. Thoma, S. Kon, T. Uede, S. F. Martin, J. C. Simon, and J. M. Weiss Osteopontin functionally activates dendritic cells and induces their differentiation toward a Th1-polarizing phenotype Blood, August 1, 2005; 106(3): 946 - 955. [Abstract] [Full Text] [PDF] M. Iwata, N. Awaya, L. Graf, C. Kahl, and B. Torok-Storb Human marrow stromal cells activate monocytes to secrete osteopontin, which down-regulates Notch1 gene expression in CD34+ cells Blood, June 15, 2004; 103(12): 4496 - 4502. [Abstract] [Full Text] [PDF] F. Shojaei, L. Gallacher, and M. Bhatia Differential gene expression of human stem progenitor cells derived from early stages of in utero human hematopoiesis Blood, April 1, 2004; 103(7): 2530 - 2540. [Abstract] [Full Text] [PDF]

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