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Prepublished online as a Blood First Edition Paper on November 21, 2002; DOI 10.1182/blood-2002-08-2438. This Article Full Text (PDF) All Versions of this Article: 2002-08-2438v1 101/7/2693    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Asnafi, V. Articles by Macintyre, E. Search for Related Content PubMed PubMed Citation Articles by Asnafi, V. Articles by Macintyre, E. Social Bookmarking                   What's this? Submitted August 9, 2002 Accepted November 3, 2002 Analysis of TCR, pT and RAG-1 in T acute lymphoblastic leukemias improves understanding of early human T lymphoid lineage commitment Vahid Asnafi, Kheira Beldjord, Emmanuelle Boulanger, Beatrice Comba, Patricia Le Tutour, Marie-Helene Estienne, Frederic Davi, Judith Landman-Parker, Pierre Quartier, Agnes Buzyn, Eric Delabesse, Francoise Valensi, and Elizabeth Macintyre* Department of Clinical and Biological Hematology, CHU / AP-HP Necker-Enfants Malades and Paris V University, Paris, France Department of Hematology, Hospital Bretonneau, Tours, France Department of Hematology, Hospital La Pitie-Salpetriere, Paris, France Department of Hematology, Hospital Trousseau, Paris, France * Corresponding author; email: elizabeth.macintyre{at}nck.ap-hop-paris.fr. T Acute Lymphoblastic Leukemias derive from human T lymphoid precursors arrested at various early stages of development. Correlation of phenotype and TCR status with RAG-1 and pT transcription in 114 T-ALLs demonstrated that they largely reflect physiological T lymphoid development. Half of the TCR lineage T-ALLs expressed a pre-TCR, as evidenced by RAG-1, pT and cTCR expression, absence of TCR deletion, and a sCD3-, CD1a+, CD4/8 DP phenotype, in keeping with a population undergoing selection. The majority of TCR T-ALLs were pT, TdT and RAG-1lo/neg, DN/SP and demonstrated only TCR DJ rearrangement, whereas 40% were pT, TdT and RAG-1 positive, DP and demonstrated TCR V(D)J rearrangement, with cTCR expression in a proportion. As such they are likely to correspond either to TCR lineage precursors selected by TCR expression or to early cells, recently derived from a pT+ common / precursor,or to a lineage deregulated / intermediate population. Approximately 30% of T-ALLs were sCD3/cTCR negative and corresponded to non-restricted thymic precursors since they expressed non T restricted markers such as CD34, CD13, CD33 and/or CD56 and were predominantly DN, CD1a, pT and RAG-1 negative, despite immature TCR and TCR rearrangements. TCR gene configuration identified progressive T lymphoid restriction. T-ALLs therefore provide homogeneous expansions of minor human lymphoid precursor populations which can clarify comprehension of normal human T cell development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: L. Terriou, R. Ben Abdelali, C. Roumier, L. Lhermitte, J. de Vos, P. Cornillet, O. Nibourel, K. Beldjord, H. Dombret, G. Leverger, et al. C/EBPA methylation is common in T-ALL but not in M0 AML Blood, February 19, 2009; 113(8): 1864 - 1866. [Full Text] [PDF] F. Armstrong, P. B. de la Grange, B. Gerby, M.-C. Rouyez, J. Calvo, M. Fontenay, N. 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