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Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-08-2588. This Article Full Text (PDF) All Versions of this Article: 2002-08-2588v1 101/6/2243    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Stringer, S. E Articles by Gupta, P. Search for Related Content PubMed PubMed Citation Articles by Stringer, S. E Articles by Gupta, P. Social Bookmarking                   What's this? Submitted August 27, 2002 Accepted October 17, 2002 Identification of a macrophage inflammatory protein-1 (MIP-1) binding heparan sulfate oligosaccharide that supports long-term in vitro maintenance of human LTC-IC Sally E Stringer, Matthew S Nelson, and Pankaj Gupta* Paterson Institute for Cancer Research, Manchester, United Kingdom Hematology/Oncology Section, VA Medical Center, Minneapolis, MN, USA Division of Hematology-Oncology-Transplantation, Department of Medicine, University of Minnesota Medical School, Minneapolis, MN, USA; Department of Genetics, Cell Biology & Development, University of Minnesota Medical School, Minneapolis, MN, USA * Corresponding author; email: gupta013{at}tc.umn.edu. We previously showed that heparan sulfate (HS) is required for in vitro, cytokine + chemokine mediated maintenance of primitive human hematopoietic progenitors. However, HS preparations are mixtures of polysaccharide chains of varying size, structure and protein-binding abilities. We therefore examined if the LTC-IC supportive capability of HS is attributable to an oligosaccharide of defined length and protein binding ability. Oligosaccharides of a wide range of sizes were prepared, and their capability to support human marrow LTC-IC maintenance in presence of low dose cytokines and a single chemokine (MIP-1) was examined. LTC-IC supportive capability of HS oligosaccharides correlated directly with size and MIP-1 binding ability. A specific, MIP-1 binding HS oligosaccharide preparation of Mr 10 kD that optimally supported LTC-IC maintenance was identified. This oligosaccharide possessed the structure required for MIP-1 binding, which we have recently described. The present study defines the minimum size and structural features of LTC-IC supportive HS. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: J. W. Murphy, Y. Cho, A. Sachpatzidis, C. Fan, M. E. Hodsdon, and E. Lolis Structural and Functional Basis of CXCL12 (Stromal Cell-derived Factor-1{alpha}) Binding to Heparin J. Biol. Chem., March 30, 2007; 282(13): 10018 - 10027. [Abstract] [Full Text] [PDF]

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