SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on January 2, 2003; DOI 10.1182/blood-2002-08-2629. This Article Full Text (PDF) All Versions of this Article: 2002-08-2629v1 101/9/3641    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Tuscano, J. M Articles by DeNardo, G. L Search for Related Content PubMed PubMed Citation Articles by Tuscano, J. M Articles by DeNardo, G. L Social Bookmarking                   What's this? Submitted August 29, 2002 Accepted December 13, 2002 The anti-CD22 ligand blocking antibody, HB22.7, has independent lymphomacidal properties and augments the efficacy of 90Y-DOTA-peptide-Lym-1 in lymphoma xenografts Joseph M Tuscano*, Robert T O'Donnell, Laird A Miers, Linda A Kroger, David L Kukis, Kathleen R Lamborn, Thomas F Tedder, and Gerald L DeNardo Department of Internal Medicine, University of California, Davis, Sacramento, CA, USA Department of Hematolog and Oncology, Verterans Administration, Northern California Health Care System, Sacramento, CA, USA Department of Immunology, Duke University, Durham, NC, USA Brain Tumor Research Center, University of California, SanFrancisco, San Francisco, CA, USA * Corresponding author; email: joseph.tuscano{at}ucdmc.ucdavis.edu. CD22 is a membrane glycophosphoprotein found on nearly all normal B-lymphocytes and most B-cell lymphomas. Recent in vitro studies have identified several anti-CD22 monoclonal antibodies (mAbs) that block the interaction of CD22 with its ligand. One of these mAbs, HB22.7, has been shown to effectively induce apoptosis in several B-cell lymphoma cell lines. Lymphoma xenograft studies with Raji-tumored mice were used to assess the toxicity and efficacy of HB22.7 both alone and with combined modality immunotherapy (CMIT) with 90Y-DOTA-peptide-Lym-1 radioimmunotherapy (RIT). The effect of the sequence of these agents on the combined treatment was assessed by administering HB22.7 24 hours prior, simultaneously, or 24 hours after RIT. Within the groups treated with RIT alone or RIT and HB22.7 (CMIT), the reduction in tumor volume was the greatest when HB22.7 was administered simultaneously and 24 hours after RIT, and in the RIT treatment groups, this translated into the greatest overall response and survival, respectively. Overall survivals at the end of the 84 day CMIT trial were 67 and 50% in the groups treated with HB22.7 simultaneously and 24 hours after RIT, respectively. This compared favorably with the untreated and RIT alone groups which had 38 and 43% surviving at the end of the trial. Surprisingly, when compared to untreated controls, and all other treatment groups, the greatest cure rate and overall survival was observed in the group treated with HB22.7 alone with 47% cured and 76% surviving at the end of the 84 day trial. RIT clearance was not affected by treatment with HB22.7. When compared to RIT alone there was no significant additional hematologic (WBC, RBC, or platelet counts) toxicity when HB22.7 was added to RIT. Non-hematologic toxicity (assessed as change in body weight) was also unchanged when HB22.7 was added to RIT. Thus the anti-CD22 ligand blocking antibody, HB22.7, has independent lymphomacidal properties, and augments the efficacy of 90Y-DOTA-peptide-Lym-1 in lymphoma xenografts, without significant toxicity. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. J. Mattes, R. M. Sharkey, H. Karacay, M. S. Czuczman, and D. M. Goldenberg Therapy of Advanced B-Lymphoma Xenografts with a Combination of 90Y-anti-CD22 IgG (Epratuzumab) and Unlabeled Anti-CD20 IgG (Veltuzumab) Clin. Cancer Res., October 1, 2008; 14(19): 6154 - 6160. [Abstract] [Full Text] [PDF] H. Tateno, H. Li, M. J. Schur, N. Bovin, P. R. Crocker, W. W. Wakarchuk, and J. C. Paulson Distinct Endocytic Mechanisms of CD22 (Siglec-2) and Siglec-F Reflect Roles in Cell Signaling and Innate Immunity Mol. Cell. Biol., August 15, 2007; 27(16): 5699 - 5710. [Abstract] [Full Text] [PDF] A. Pantelias, J. M. Pagel, N. Hedin, L. Saganic, S. Wilbur, D. K. Hamlin, D. S. Wilbur, Y. Lin, D. Stone, D. Axworthy, et al. Comparative biodistributions of pretargeted radioimmunoconjugates targeting CD20, CD22, and DR molecules on human B-cell lymphomas Blood, June 1, 2007; 109(11): 4980 - 4987. [Abstract] [Full Text] [PDF] K. M. Haas, S. Sen, I. G. Sanford, A. S. Miller, J. C. Poe, and T. F. Tedder CD22 Ligand Binding Regulates Normal and Malignant B Lymphocyte Survival In Vivo. J. Immunol., September 1, 2006; 177(5): 3063 - 3073. [Abstract] [Full Text] [PDF] M. Ho, S. Nagata, and I. Pastan Isolation of anti-CD22 Fv with high affinity by Fv display on human cells PNAS, June 20, 2006; 103(25): 9637 - 9642. [Abstract] [Full Text] [PDF] A. Varki and T. Angata Siglecs--the major subfamily of I-type lectins Glycobiology, January 1, 2006; 16(1): 1R - 27R. [Abstract] [Full Text] [PDF] D. A. Vallera, M. W. Brechbiel, L. J. Burns, A. Panoskaltsis-Mortari, K. E. Dusenbery, D. R. Clohisy, and E. S. Vitetta Radioimmunotherapy of CD22-Expressing Daudi Tumors in Nude Mice with a 90Y-Labeled Anti-CD22 Monoclonal Antibody Clin. Cancer Res., November 1, 2005; 11(21): 7920 - 7928. [Abstract] [Full Text] [PDF] M. Zhang and A. Varki Cell surface sialic acids do not affect primary CD22 interactions with CD45 and surface IgM nor the rate of constitutive CD22 endocytosis Glycobiology, November 1, 2004; 14(11): 939 - 949. [Abstract] [Full Text] [PDF] M. J.S. Dyer Bioweapons of Tumor Mass Destruction? J. Clin. Oncol., August 15, 2003; 21(16): 3011 - 3012. [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020