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Prepublished online as a Blood First Edition Paper on November 7, 2002; DOI 10.1182/blood-2002-08-2663. This Article Full Text (PDF) All Versions of this Article: 2002-08-2663v1 101/6/2199    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Hematti, P. Articles by Dunbar, C. E Search for Related Content PubMed PubMed Citation Articles by Hematti, P. Articles by Dunbar, C. E Social Bookmarking                   What's this? Submitted August 30, 2002 Accepted November 1, 2002 Retroviral transduction efficiency of G-CSF+SCF mobilized peripheral blood CD34+ cells is superior to G-CSF or G-CSF+Flt3-L mobilized cells in nonhuman primates Peiman Hematti, Stephanie E Sellers, Brian A Agricola, Mark E Metzger, Robert E Donahue, and Cynthia E Dunbar* National Institutes of Health, Hematology Branch, NHLBI, Bethesda, MD, USA * Corresponding author; email: dunbarc{at}nhlbi.nih.gov. Gene transfer experiments in nonhuman primates have been shown to be predictive of success in human clinical gene therapy trials. In most nonhuman primate studies, hematopoietic stem cells (HSCs) collected from the peripheral blood or bone marrow after administration of G-CSF+stem cell factor (SCF) have been used as targets, but this cytokine combination is not generally available for clinical use, and the optimum target cell population has not been systematically studied. In our current study we tested the retroviral transduction efficiency of rhesus macaque peripheral blood CD34+ cells collected after administration of different cytokine mobilization regimens, directly comparing G-CSF+SCF versus G-CSF alone or G-CSF+Flt3-L in competitive repopulation assays. Vector supernatant was added daily for 96 hours in the presence of stimulatory cytokines. The transduction efficiency of HSC as assessed by in vitro colony forming assays was equivalent in all five animals tested, but the in vivo levels of mononuclear cell and granulocyte marking was higher at all time points derived from target CD34+ cells collected after G-CSF+SCF mobilization compared to target cells collected after G-CSF (n=3) or G-CSF+Flt3-L (n=2) mobilization. In three of the animals long-term marking levels of 5-25% was achieved, but only originating from the G-CSF+SCF mobilized target cells. Transduction efficiency of HSCs collected by different mobilization regimens can vary significantly, and is superior with G-CSF+SCF administration. The difference in transduction efficiency of HSCs collected from different sources should be considered whenever planning clinical gene therapy trials and preferably be tested directly in comparative studies. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: B. E. Shepherd, H.-P. Kiem, P. M. Lansdorp, C. E. Dunbar, G. Aubert, A. LaRochelle, R. Seggewiss, P. Guttorp, and J. L. Abkowitz Hematopoietic stem-cell behavior in nonhuman primates Blood, September 15, 2007; 110(6): 1806 - 1813. [Abstract] [Full Text] [PDF] A. Larochelle, A. Krouse, M. Metzger, D. Orlic, R. E. Donahue, S. Fricker, G. Bridger, C. E. Dunbar, and P. 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