SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on October 24, 2002; DOI 10.1182/blood-2002-09-2742. This Article Full Text (PDF) All Versions of this Article: 2002-09-2742v1 101/5/2008    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Vogt, T. M Articles by Enns, C. A Search for Related Content PubMed PubMed Citation Articles by Vogt, T. M Articles by Enns, C. A Social Bookmarking                   What's this? Submitted September 9, 2002 Accepted October 16, 2002 Heterotypic interactions between Transferrin Receptor and Transferrin Receptor 2 Todd M Vogt, Aaron D Blackwell, Anthony M Giannetti, Pamela J Bjorkman, and Caroline A Enns* Department of Cell and Developmental Biology, Oregon Health & Science University, Portland, OR, USA Divison of Biology, California Institute of Technology, Pasadena, CA, USA * Corresponding author; email: ennsca{at}ohsu.edu. Cellular iron uptake in most tissues occurs via endocytosis of diferric transferrin (Tf) bound to the transferrin receptor (TfR). Recently, a second Tf receptor, transferrin receptor 2 (TfR2), has been identified and shown to play a critical role in iron metabolism. TfR2 is capable of Tf-mediated iron uptake and mutations in this gene result in a rare form of hereditary hemochromatosis unrelated to the hereditary hemochromatosis protein, HFE. Unlike TfR, TfR2 expression is not controlled by cellular iron concentrations and little information is currently available regarding the role of TfR2 in cellular iron homeostasis. To investigate the relationship between TfR and TfR2, we performed a series of in vivo and in vitro experiments using antibodies generated to each receptor. Western blots demonstrate that TfR2 protein is expressed strongest in erythroid cell lines. Metabolic labeling studies indicate that TfR2 protein levels are approximately 20-fold lower than TfR in these cells. TfR and TfR2 have similar cellular localizations in K562 cells and co-immunoprecipitate only to a very limited extent. Western analysis of the receptors under nonreducing conditions reveals that they can form heterodimers. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: C. E. Herbison, K. Thorstensen, A. C. G. Chua, R. M. Graham, P. Leedman, J. K. Olynyk, and D. Trinder The role of transferrin receptor 1 and 2 in transferrin-bound iron uptake in human hepatoma cells Am J Physiol Cell Physiol, December 1, 2009; 297(6): C1567 - C1575. [Abstract] [Full Text] [PDF] J. Chen, M. Chloupkova, J. Gao, T. L. Chapman-Arvedson, and C. A. Enns HFE Modulates Transferrin Receptor 2 Levels in Hepatoma Cells via Interactions That Differ from Transferrin Receptor 1-HFE Interactions J. Biol. Chem., December 21, 2007; 282(51): 36862 - 36870. [Abstract] [Full Text] [PDF] M. B. Johnson, J. Chen, N. Murchison, F. A. Green, and C. A. Enns Transferrin Receptor 2: Evidence for Ligand-induced Stabilization and Redirection to a Recycling Pathway Mol. Biol. Cell, March 1, 2007; 18(3): 743 - 754. [Abstract] [Full Text] [PDF] I. C. Moura, M. Arcos-Fajardo, A. Gdoura, V. Leroy, C. Sadaka, N. Mahlaoui, Y. Lepelletier, F. Vrtovsnik, E. Haddad, M. Benhamou, et al. Engagement of Transferrin Receptor by Polymeric IgA1: Evidence for a Positive Feedback Loop Involving Increased Receptor Expression and Mesangial Cell Proliferation in IgA Nephropathy J. Am. Soc. Nephrol., September 1, 2005; 16(9): 2667 - 2676. [Abstract] [Full Text] [PDF] X. Xu, H. L. Persson, and D. R. Richardson Molecular Pharmacology of the Interaction of Anthracyclines with Iron Mol. Pharmacol., August 1, 2005; 68(2): 261 - 271. [Abstract] [Full Text] [PDF] V. Decot, G. Woerly, M. Loyens, S. Loiseau, B. Quatannens, M. Capron, and D. Dombrowicz Heterogeneity of Expression of IgA Receptors by Human, Mouse, and Rat Eosinophils J. Immunol., January 15, 2005; 174(2): 628 - 635. [Abstract] [Full Text] [PDF] M. B. Johnson and C. A. Enns Diferric transferrin regulates transferrin receptor 2 protein stability Blood, December 15, 2004; 104(13): 4287 - 4293. [Abstract] [Full Text] [PDF] A. Robb and M. Wessling-Resnick Regulation of transferrin receptor 2 protein levels by transferrin Blood, December 15, 2004; 104(13): 4294 - 4299. [Abstract] [Full Text] [PDF] A. D. Robb, M. Ericsson, and M. Wessling-Resnick Transferrin receptor 2 mediates a biphasic pattern of transferrin uptake associated with ligand delivery to multivesicular bodies Am J Physiol Cell Physiol, December 1, 2004; 287(6): C1769 - C1775. [Abstract] [Full Text] [PDF] I. C. Moura, M. Arcos-Fajardo, C. Sadaka, V. Leroy, M. Benhamou, J. Novak, F. Vrtovsnik, E. Haddad, K. R. Chintalacharuvu, and R. C. Monteiro Glycosylation and Size of IgA1 Are Essential for Interaction with Mesangial Transferrin Receptor in IgA Nephropathy J. Am. Soc. Nephrol., March 1, 2004; 15(3): 622 - 634. [Abstract] [Full Text] [PDF]

	Copyright © 2002 by American Society of Hematology         Online ISSN: 1528-0020