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Prepublished online as a Blood First Edition Paper on January 9, 2003; DOI 10.1182/blood-2002-09-2767. This Article Full Text (PDF) All Versions of this Article: 2002-09-2767v1 101/9/3460    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Khanna-Gupta, A. Articles by Berliner, N. Search for Related Content PubMed PubMed Citation Articles by Khanna-Gupta, A. Articles by Berliner, N. Social Bookmarking                   What's this? Submitted September 10, 2002 Accepted December 18, 2002 Chromatin immunoprecipitation (ChIP) studies indicate a role for CCAAT enhancer binding proteins alpha and epsilon (C/EBP and C/EBP) and CDP/cut in myeloid maturation-induced lactoferrin gene expression Arati Khanna-Gupta, Theresa Zibello, Hong Sun, Peter Gaines, and Nancy Berliner* Department of Internal Medicine/Hematology, Yale University, New Haven, CT, USA * Corresponding author; email: nancy.berliner{at}yale.edu. In vitro models of granulopoiesis involving the inducible expression of either C/EBP or C/EBP in myeloid cells have been shown to lead to the induction of a granulocytic maturation program accompanied by the expression of myeloid specific genes. Since members of the C/EBP family of transcription factors recognize and bind to similar DNA-binding motifs, it has been difficult to elucidate the specific role of each of the C/EBP family members in eliciting myeloid gene expression. In order to address this issue, we focused on the expression of the lactoferrin (LF) gene. LF expression is transcriptionally regulated in a C/EBP- dependent manner in myeloid cells. Using ChIP analysis we demonstrate that C/EBP binds to the LF promoter in non-expressing cells. Upon induction of maturation, C/EBP binds to the LF promoter, which correlates with LF expression. Lack of LF expression in the acute promyelocytic leukemia cell line NB4, which harbors the t(15;17) translocation, cannot be correlated with aberrant binding at the C/EBP site in the LF promoter. It is however associated with the persistent binding of the silencer CDP/cut to the LF promoter in these cells. We conclude that C/EBP, C/EBP and CDP/cut all play definitive roles in regulating late gene expression during normal myeloid development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. Moriuchi and H. Moriuchi Induction of Lactoferrin Gene Expression in Myeloid or Mammary Gland Cells by Human T-Cell Leukemia Virus Type 1 (HTLV-1) Tax: Implications for Milk-Borne Transmission of HTLV-1 J. Virol., July 15, 2006; 80(14): 7118 - 7126. [Abstract] [Full Text] [PDF] A. Khanna-Gupta, H. Sun, T. Zibello, L. Lozovatsky, P. K. Ghosh, D. C. Link, M. L. McLemore, and N. Berliner p120 nucleolar-proliferating antigen is a direct target of G-CSF signaling during myeloid differentiation J. Leukoc. Biol., May 1, 2006; 79(5): 1011 - 1021. [Abstract] [Full Text] [PDF] A. F. Gombart, U. Krug, J. O'Kelly, E. An, V. Vegesna, and H. P. Koeffler Aberrant expression of neutrophil and macrophage-related genes in a murine model for human neutrophil-specific granule deficiency J. Leukoc. Biol., November 1, 2005; 78(5): 1153 - 1165. [Abstract] [Full Text] [PDF] J. J. Krupp, L. E. Yaich, R. J. Wessells, and R. Bodmer Identification of Genetic Loci That Interact With cut During Drosophila Wing-Margin Development Genetics, August 1, 2005; 170(4): 1775 - 1795. [Abstract] [Full Text] [PDF] J. Dong, S. Fujii, H. Li, H. Nakabayashi, M. Sakai, S. Nishi, D. Goto, T. Furumoto, S. Imagawa, T. A.K.M. Zaman, et al. Interleukin-6 and Mevastatin Regulate Plasminogen Activator Inhibitor-1 Through CCAAT/Enhancer-Binding Protein-{delta} Arterioscler Thromb Vasc Biol, May 1, 2005; 25(5): 1078 - 1084. [Abstract] [Full Text] [PDF] Q. Zhu, U. Maitra, D. Johnston, M. Lozano, and J. P. Dudley The Homeodomain Protein CDP Regulates Mammary-Specific Gene Transcription and Tumorigenesis Mol. Cell. Biol., June 1, 2004; 24(11): 4810 - 4823. [Abstract] [Full Text] [PDF] N. A. Maun, P. Gaines, A. Khanna-Gupta, T. Zibello, L. Enriquez, L. Goldberg, and N. Berliner G-CSF signaling can differentiate promyelocytes expressing a defective retinoic acid receptor: evidence for divergent pathways regulating neutrophil differentiation Blood, March 1, 2004; 103(5): 1693 - 1701. [Abstract] [Full Text] [PDF]

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