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Prepublished online as a Blood First Edition Paper on March 6, 2003; DOI 10.1182/blood-2002-09-2831. This Article Full Text (PDF) All Versions of this Article: 2002-09-2831v1 102/2/731    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Wolff, J. Articles by Bux, J. Search for Related Content PubMed PubMed Citation Articles by Wolff, J. Articles by Bux, J. Social Bookmarking                   What's this? Submitted September 19, 2002 Accepted February 11, 2003 Lack of NB1 GP (CD177 / HNA-2a) gene transcription in NB1 GP-negative neutrophils from NB1 GP-expressing individuals and association of low expression with NB1 gene polymorphisms Jens Wolff, Cornelia Brendel, Ludger Fink, Rainer M Bohle, Karin Kissel, and Juergen Bux* Institute for Clinical Immunology and Transfusion Medicine, University of Giessen, Giessen, Germany Department of Hematology, University of Marburg, Marburg, Germany Institute for Pathology, University of Giessen, Giessen, Germany * Corresponding author; email: Juergen.Bux{at}immunologie.med.uni-giessen.de. The human neutrophil NB1 glycoprotein (NB1 GP, HNA-2a, CD177) has gained clinical importance being involved in TRALI reactions and immune neutropenias. The NB1 GP shows the unique feature of being expressed only on a neutrophil subpopulation. Recently, we identified splicing defects responsible for a NB1 GP deficiency. In this study, we have investigated the molecular basis of the heterogeneous expression of NB1 GP by separating the two neutrophil subpopulations using immunofluorescence followed by single cell picking or by fluorescence-activated cell sorter, respectively. We found a lack of NB1 mRNA in the NB1 GP-negative cells that remained constant even after G-CSF administration. Comparing the cDNA sequences of donors with a large (> 60%) and those with a small (< 40%) NB1 GP-expressing subpopulation, we found six polymorphisms. Three of them were significantly associated with a small NB1 GP-expressing subpopulation indicating a genetic basis for NB1 GP non-expression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: A. Drewniak, J.-J. Boelens, H. Vrielink, A. T.J. Tool, M. C.A. Bruin, M. van den Heuvel-Eibrink, L. Ball, M. D. van de Wetering, D. Roos, and T. W. Kuijpers Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes Haematologica, July 1, 2008; 93(7): 1058 - 1067. [Abstract] [Full Text] [PDF] S. von Vietinghoff, G. Tunnemann, C. Eulenberg, M. Wellner, M. Cristina Cardoso, F. C. Luft, and R. Kettritz NB1 mediates surface expression of the ANCA antigen proteinase 3 on human neutrophils Blood, May 15, 2007; 109(10): 4487 - 4493. [Abstract] [Full Text] [PDF] U. J. H. Sachs, K. Hattar, N. Weissmann, R. M. Bohle, T. Weiss, U. Sibelius, and J. Bux Antibody-induced neutrophil activation as a trigger for transfusion-related acute lung injury in an ex vivo rat lung model Blood, February 1, 2006; 107(3): 1217 - 1219. [Abstract] [Full Text] [PDF]

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