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Prepublished online as a Blood First Edition Paper on April 3, 2003; DOI 10.1182/blood-2002-09-2913. This Article Full Text (PDF) All Versions of this Article: 2002-09-2913v1 102/3/1000    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Asnafi, V. Articles by Macintyre, E. Search for Related Content PubMed PubMed Citation Articles by Asnafi, V. Articles by Macintyre, E. Social Bookmarking                   What's this? Submitted September 24, 2002 Accepted March 22, 2003 CALM-AF10 is a common fusion transcript in T-ALL and is specific to the TCR lineage Vahid Asnafi, Isabelle Radford-Weiss, Nicole Dastugue, Chantal Bayle, Daniel Leboeuf, Christiane Charrin, Richard Garand, Marina Lafage-Pochitaloff, Eric Delabesse, Agnes Buzyn, Xavier Troussard, and Elizabeth Macintyre* Laboratoire d'Hematologie, CHU Necker Enfants Malades and University Paris V, Paris, France Service d'Histologie, Embryologie, et de Cytogenetic, CHU Necker Enfants Maladesand University Paris V, Paris, France Laboratoire d'Hematologie et Genetique des Hemopathies, CHU Purpan, Toulouse, France Laboratoire d'Hematologie, Institut Gustave Roussy, Villejuif, France Laboratoire d'Hematologie, CHU Edouard Herriot, Lyon, France Institut de Biologie des Hopitaux, CHU Nantes, Nantes, France Departement de Biologie, Unite de Genetique Hematologique, Institut Paoli Calmette, Marseille, France Service d'Hematologie Clinique, CHU Necker Enfants Malades, Paris, France Laboratoire d'Hematologie, CHU Caen, Caen, France * Corresponding author; email: elizabeth.macintyre{at}nck.ap-hop-paris.fr. The t(10;11)(p13-14;q14-21) associated with CALM-AF10 is considered to be rare and associated with a variety of acute lymphoid and myeloid leukemias. Twelve (9%) of 131 unselected T-ALLs expressed CALM-AF10 by RT-PCR and/or FISH, including 8% of children and 10% of adults, of which only half demonstrated a t(10;11) by classical cytogenetics. CALM-AF10 was not found in TCR lineage T-ALLs, as defined by expression of TCR, cytTCR and/or TCR VDJ rearrangement in immature (IM) cytTCR- cases, compared to 19% of TCR T-ALLs and 33% of IM/ T-ALLs. The latter differed from their CALM-AF10 negative immature counterparts by a CD5+/CD2- phenotype, as found in TCR but not TCR T-ALLs and in their TCR and TCR configurations, altogether suggesting that CALM-AF10+ IM/ T-ALLs are TCR precursors and that, within T-ALL, CALM-AF10 is specific for this lineage. Nine of 12 immature CALM-AF10 T-ALLs demonstrated 3' fusion transcripts, whereas 6/7 TCR T-ALLs demonstrated 5' fusion transcripts. The latter retain the AF10 extended LAP/PHD domain necessary for homo-oligomerisation. All 8 CALM-AF10+ TCR T-ALLs are alive, compared to only 3/12 immature CALM-AF10+ T-ALLs. Six CALM-AF10+ non-T acute leukemias all expressed CD7 and demonstrated T restricted TCR rearrangements, suggesting that they may also be related to the TCR lineage. CALM-AF10 is therefore the most common fusion protein in T-ALL. It requires molecular and immunophenotypic characterization for appropriate prognostic evaluation and should be included in diagnostic screening panels of T-ALL and immature AL. Analysis of immature CALM-AF10+ leukemias will also facilitate analysis of the early stages of TCR lineage development. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: F. Baleydier, A.-V. Decouvelaere, J. Bergeron, P. Gaulard, D. Canioni, Y. Bertrand, S. Lepretre, B. Petit, H. Dombret, K. Beldjord, et al. 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