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Prepublished online as a Blood First Edition Paper on December 12, 2002; DOI 10.1182/blood-2002-09-2924. This Article Full Text (PDF) All Versions of this Article: 2002-09-2924v1 101/8/3058    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Gobin, S. J Articles by Van den Elsen, P. J Search for Related Content PubMed PubMed Citation Articles by Gobin, S. J Articles by Van den Elsen, P. J Social Bookmarking                   What's this? Submitted September 25, 2002 Accepted November 27, 2002 Regulation of human 2-microglobulin transactivation in hematopoietic cells Sam J Gobin*, Paula Biesta, and Peter J Van den Elsen Immunohematology and Blood Transfusion, Leiden University Medical Center (LUMC), Leiden, The Netherlands * Corresponding author; email: gobin{at}lumc.nl. 2-microglobulin (2m) is a chaperone of major histocompatibility complex (MHC) class I (-like) molecules that play a central role in antigen presentation, immunoglobulin transport and iron metabolism. It is therefore of importance that 2m is adequately expressed in cells that perform these functions, such as hematopoietic cells. In this study we investigated the transcriptional regulation of 2m in lymphoid and myeloid cell lines through a promoter containing a putative E box, Ets/interferon-stimulated response element (ISRE) and B site. Here we show that USF1 and USF2 bind to the E box and regulate 2m transactivation. The nuclear factor B (NF-B) subunits p50 and p65 bind to the B box and p65 transactivates 2m. Interferon regulatory factor 1 (IRF1), IRF2, IRF4 and IRF8, but not PU.1, bind to the ISRE and IRF1 and IRF3 are strong transactivators of b2m. Together, all three boxes are important for the constitutive and cytokine-induced levels of 2m expression in lymphoid and myeloid cell types. As such, b2m transactivation is under the control of important transcriptional pathways, which are activated during injury, infection and inflammation. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: H. F. Kuipers, P. J. Biesta, L. J. Montagne, E. S. van Haastert, P. van der Valk, and P. J. van den Elsen CC chemokine receptor 5 gene promoter activation by the cyclic AMP response element binding transcription factor Blood, September 1, 2008; 112(5): 1610 - 1619. [Abstract] [Full Text] [PDF] S. Zhang, V. Metelev, D. Tabatadze, P. C. Zamecnik, and A. Bogdanov Jr Fluorescence resonance energy transfer in near-infrared fluorescent oligonucleotide probes for detecting protein-DNA interactions PNAS, March 18, 2008; 105(11): 4156 - 4161. [Abstract] [Full Text] [PDF] T. M. Holling, M. W. T. Bergevoet, L. Wilson, M. C. J. A. Van Eggermond, E. Schooten, R. D. M. Steenbergen, P. J. F. Snijders, M. J. Jager, and P. J. Van den Elsen A Role for EZH2 in Silencing of IFN-{gamma} Inducible MHC2TA Transcription in Uveal Melanoma J. Immunol., October 15, 2007; 179(8): 5317 - 5325. [Abstract] [Full Text] [PDF] X. Liu, L. Ye, G. J. Christianson, J.-Q. Yang, D. C. Roopenian, and X. Zhu NF-{kappa}B Signaling Regulates Functional Expression of the MHC Class I-Related Neonatal Fc Receptor for IgG via Intronic Binding Sequences J. Immunol., September 1, 2007; 179(5): 2999 - 3011. [Abstract] [Full Text] [PDF] A. Grassadonia, N. Tinari, B. Fiorentino, M. Nakazato, H.-K. Chung, C. Giuliani, G. Napolitano, S. Iacobelli, T. K. Howcroft, D. S. Singer, et al. Upstream Stimulatory Factor Regulates Constitutive Expression and Hormonal Suppression of the 90K (Mac-2BP) Protein Endocrinology, July 1, 2007; 148(7): 3507 - 3517. [Abstract] [Full Text] [PDF] H. K. Bayele, H. McArdle, and S. K.S. Srai Cis and trans regulation of hepcidin expression by upstream stimulatory factor Blood, December 15, 2006; 108(13): 4237 - 4245. [Abstract] [Full Text] [PDF] A. Zeold, M. Doleschall, M. C. Haffner, L. P. Capelo, J. Menyhert, Z. Liposits, W. S. da Silva, A. C. Bianco, I. Kacskovics, C. Fekete, et al. Characterization of the Nuclear Factor-{kappa}B Responsiveness of the Human dio2 Gene Endocrinology, September 1, 2006; 147(9): 4419 - 4429. [Abstract] [Full Text] [PDF] Y. Okada, E. Matsuura, Z. Tozuka, R. Nagai, A. Watanabe, K. Matsumoto, K. Yasui, R. W. Jackman, T. Nakano, and T. Doi Upstream stimulatory factors stimulate transcription through E-box motifs in the PF4 gene in megakaryocytes Blood, October 1, 2004; 104(7): 2027 - 2034. [Abstract] [Full Text] [PDF]

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