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Prepublished online as a Blood First Edition Paper on June 26, 2003; DOI 10.1182/blood-2002-09-2929. This Article Full Text (PDF) All Versions of this Article: 2002-09-2929v1 102/7/2513    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Coates, P. T Articles by Thomson, A. W Search for Related Content PubMed PubMed Citation Articles by Coates, P. T Articles by Thomson, A. W Social Bookmarking                   What's this? Submitted September 26, 2002 Accepted May 23, 2003 Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt3 ligand Patrick T Coates, Simon M Barratt-Boyes, Linyou Zhang, Vera S Donnenberg, Peta J O'Connell, Alison J Logar, F J Duncan, Michael Murphey-Corb, Albert D Donnenberg, Adrian E Morelli, Charles Maliszewski, and Angus W Thomson* Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA, USA; Immunology, University of Pittsburgh, Pittsburgh, PA, USA Medicine, University of Pittsburgh, Pittsburgh, PA, USA Infectious Diseases and Microbiology, University of Pittsburgh, Pittsburgh, PA, USA; Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA, USA Immunex Corporation, Seattle, WA, USA Thomas E Starzl Transplantation Institute, University of Pittsburgh, Pittsburgh, PA, USA; Molecular Genetics and Biochemistry, University of Pittsburgh, Pittsburgh, PA, USA; Immunology, University of Pittsburgh, Pittsburgh, PA, USA * Corresponding author; email: thomsonaw{at}msx.upmc.edu. We provide phenotypic and functional evidence of pre-monocytoid DC and pre-plasmacytoid DC in blood and of corresponding DC subsets in secondary lymphoid tissue of rhesus monkeys. Subsets were identified and sorted by 4-color flow cytometry using anti-human monoclonal antibodies cross-reactive with rhesus monkey. To mobilize pre-DC subsets, fms-like tyrosine 3 kinase ligand (Flt3L ;100µg/kg s.c.) was administered for 10 days. Presumptive pre-DC subsets were identified within the lineage-MHC class II+ fraction of blood mononuclear cells. Pre-monocytoid DC were CD11c+ CD123-( IL-3Ra-); pre-plasmacytoid DC were characterized as CD11c- CD123++. Flt3L increased the CD11c+ pre-DC (7-fold) and CD123++ pre-DC subsets (3-fold) in blood. The freshly-isolated CD11c+ pre-DC subset induced modest proliferation of naive allogeneic T cells. After overnight culture with GM-CSF and CD40L, both subsets upregulated surface costimulatory molecules and CD11c+ pre-DC became potent allostimulators. Freshly-isolated CD123++ pre-DC showed typical plasmacytoid morphology and when cultured with IL-3 and CD40L for 72 hr, developed mature DC morphology. Following stimulation with CD40L, CD11c+ pre-DC secreted increased levels of IL-12p40. Importantly, herpes simplex virus-stimulated CD123++ pre-DC but not CD11c+pre-DC secreted interferon- (IFN-). Corresponding DC subsets were identified by flow analysis and immunohistochemistry in lymph nodes wherein both populations were increased 2-3 fold by Flt3L administration. CD123+ pre-DC produced IFN- in response to in vivo viral infection. Thus, rhesus monkeys exhibit two distinct DC precursor populations that closely resemble those of humans. Both are mobilized into blood and lymphoid tissue by Flt3L, offering potential for their further characterization and possible therapeutic application. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: S. Agrawal, P. 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