SEARCH:   Advanced

Prepublished online as a Blood First Edition Paper on January 30, 2003; DOI 10.1182/blood-2002-10-3139. This Article Full Text (PDF) All Versions of this Article: 2002-10-3139v1 101/11/4342    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cotta, C. V Articles by Klug, C. A Search for Related Content PubMed PubMed Citation Articles by Cotta, C. V Articles by Klug, C. A Social Bookmarking                   What's this? Submitted October 17, 2002 Accepted January 23, 2003 Pax5 determines B versus T cell fate and does not block early myeloid-lineage development Claudiu V Cotta, Zheng Zhang, Hyung-Gyoon Kim, and Christopher A Klug* Department of Pathology, University of Alabama at Birmingham, Birmingham, AL, USA Department of Microbiology, University of Alabama at Birmingham, Birmingham, AL, USA Division of Developmental and Clinical Immunology, University of Alabama at Birmingham, Birmingham, AL, USA * Corresponding author; email: chris.klug{at}ccc.uab.edu. Progenitor B cells deficient in Pax5 are developmentally multipotent, suggesting that Pax5 is necessary to maintain commitment to the B cell lineage. Commitment may be mediated, in part, by Pax5 repression of myeloid-specific genes. To determine whether Pax5 expression in multipotential cells is sufficient to restrict development to the B cell lineage in vivo, we enforced expression of Pax5 in hematopoietic stem cells using a retroviral vector. Peripheral blood analysis of all animals reconstituted with Pax5-expressing cells indicated that greater than 90 percent of Pax5-expressing cells were B220+ mature B cells that were not malignant. Further analysis showed that Pax5 completely blocked T lineage development in the thymus but did not inhibit myelopoiesis or NK cell development in bone marrow. These results implicate Pax5 as a critical regulator of B versus T cell developmental fate and suggest that Pax5 may promote commitment to the B cell lineage by mechanisms that are independent of myeloid gene repression. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: M. A. Thal, T. L. Carvalho, T. He, H.-G. Kim, H. Gao, J. Hagman, and C. A. Klug Ebf1-mediated down-regulation of Id2 and Id3 is essential for specification of the B cell lineage PNAS, January 13, 2009; 106(2): 552 - 557. [Abstract] [Full Text] [PDF] H.-G. Kim, K. Kojima, C. S. Swindle, C. V. Cotta, Y. Huo, V. Reddy, and C. A. Klug FLT3-ITD cooperates with inv(16) to promote progression to acute myeloid leukemia Blood, February 1, 2008; 111(3): 1567 - 1574. [Abstract] [Full Text] [PDF] G. Fazio, C. Palmi, A. Rolink, A. Biondi, and G. Cazzaniga PAX5/TEL Acts as a Transcriptional Repressor Causing Down-modulation of CD19, Enhances Migration to CXCL12, and Confers Survival Advantage in pre-BI Cells Cancer Res., January 1, 2008; 68(1): 181 - 189. [Abstract] [Full Text] [PDF] K. Anderson, C. Rusterholz, R. Mansson, C. T. Jensen, K. Bacos, S. Zandi, Y. Sasaki, C. Nerlov, M. Sigvardsson, and S. E. W. Jacobsen Ectopic expression of PAX5 promotes maintenance of biphenotypic myeloid progenitors coexpressing myeloid and B-cell lineage-associated genes Blood, May 1, 2007; 109(9): 3697 - 3705. [Abstract] [Full Text] [PDF] Z. Zhang, C. S. Swindle, J. T. Bates, R. Ko, C. V. Cotta, and C. A. Klug Expression of a non-DNA-binding isoform of Helios induces T-cell lymphoma in mice Blood, March 1, 2007; 109(5): 2190 - 2197. [Abstract] [Full Text] [PDF] C.-L. Hsu, A. G. King-Fleischman, A. Y. Lai, Y. Matsumoto, I. L. Weissman, and M. Kondo Antagonistic effect of CCAAT enhancer-binding protein-{alpha} and Pax5 in myeloid or lymphoid lineage choice in common lymphoid progenitors PNAS, January 17, 2006; 103(3): 672 - 677. [Abstract] [Full Text] [PDF] S. Dovat, E. Montecino-Rodriguez, V. Schuman, M. A. Teitell, K. Dorshkind, and S. T. Smale Transgenic Expression of Helios in B Lineage Cells Alters B Cell Properties and Promotes Lymphomagenesis J. Immunol., September 15, 2005; 175(6): 3508 - 3515. [Abstract] [Full Text] [PDF] C. A. Goetz, I. R. Harmon, J. J. O'Neil, M. A. Burchill, T. M. Johanns, and M. A. Farrar Restricted STAT5 Activation Dictates Appropriate Thymic B versus T Cell Lineage Commitment J. Immunol., June 15, 2005; 174(12): 7753 - 7763. [Abstract] [Full Text] [PDF] H. Min, E. Montecino-Rodriguez, and K. Dorshkind Reduction in the Developmental Potential of Intrathymic T Cell Progenitors with Age J. Immunol., July 1, 2004; 173(1): 245 - 250. [Abstract] [Full Text] [PDF]

	Copyright © 2003 by American Society of Hematology         Online ISSN: 1528-0020