|
|
Prepublished online as a Blood First Edition Paper on March 6, 2003; DOI 10.1182/blood-2002-10-3159.
Submitted November 7, 2002
Accepted February 19, 2003
LFA-1 is required for retention of effector CD8 T cells in mouse lungs
Jayant Thatte, Vrushali Dabak, Mark B Williams, Thomas J Braciale, and Klaus Ley*
Department of Biomedical Engineering, University of Virginia, Charlottesville, VA, USA
* Corresponding author; email: klausley{at}virginia.edu.
The adhesion molecules involved in the migration and retention of activated effector CD8 T cells in the lung microcirculation and their recruitment into lung tissue are largely unknown. Here, we have analyzed the role of LFA-1 and VLA-4 on adhesion of influenza hemagglutinin (HA)-specific CD8 T cell clone D4 under shear conditions in an in vitro binding assay and in an in vivo homing assay to the lungs of naive or transgenic Balb/c mice expressing HA (HA-Tg) by a lung-specific promoter. Blocking LFA-1 or ICAM-1 significantly inhibited adhesion of D4 cells to lung vascular endothelium and parenchyma of lung sections. However, blocking VLA-4 or VCAM-1 had no effect on cell adhesion. Blocking LFA-1 in vivo significantly delayed lethal injury following adoptive transfer of D4 cells into HA-Tg mice as assessed by weight loss and histology. Residence time of adoptively transferred 111In-labeled D4 cells in lungs of normal and HA-Tg mice as analyzed by dual modality imaging revealed a significantly shorter transit time of 4 hours for the D4 cells upon in vivo blockade of LFA-1. These results demonstrate a crucial role for LFA-1 in retention of activated CD8 T cells in normal mouse lungs and in the progression of lethal injury in HA-Tg mice.
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati What's this?
This article has been cited by other articles:
|
|
|
|
 
G. H. Y. Lin, B. J. Sedgmen, T. J. Moraes, L. M. Snell, D. J. Topham, and T. H. Watts
Endogenous 4-1BB Ligand Plays a Critical Role in Protection from Influenza-Induced Disease
J. Immunol.,
January 15, 2009;
182(2):
934 - 947.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. K. Polakos, I. Klein, M. V. Richter, D. M. Zaiss, M. Giannandrea, I. N. Crispe, and D. J. Topham
Early Intrahepatic Accumulation of CD8+ T Cells Provides a Source of Effectors for Nonhepatic Immune Responses
J. Immunol.,
July 1, 2007;
179(1):
201 - 210.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Zhang, E. P. Wojcikiewicz, and V. T. Moy
Dynamic Adhesion of T Lymphocytes to Endothelial Cells Revealed by Atomic Force Microscopy
Experimental Biology and Medicine,
September 1, 2006;
231(8):
1306 - 1312.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Basit, J. Reutershan, M. A. Morris, M. Solga, C. E. Rose Jr., and K. Ley
ICAM-1 and LFA-1 play critical roles in LPS-induced neutrophil recruitment into the alveolar space
Am J Physiol Lung Cell Mol Physiol,
August 1, 2006;
291(2):
L200 - L207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Ghosh, A. A. Chackerian, C. M. Parker, C. M. Ballantyne, and S. M. Behar
The LFA-1 adhesion molecule is required for protective immunity during pulmonary Mycobacterium tuberculosis infection.
J. Immunol.,
April 15, 2006;
176(8):
4914 - 4922.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. Whitmire, N. Benning, and J. L. Whitton
Precursor Frequency, Nonlinear Proliferation, and Functional Maturation of Virus-Specific CD4+ T Cells.
J. Immunol.,
March 1, 2006;
176(5):
3028 - 3036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Schreiber, S. Ehlers, S. Aly, A. Holscher, S. Hartmann, M. Lipp, J. B. Lowe, and C. Holscher
Selectin Ligand-Independent Priming and Maintenance of T Cell Immunity during Airborne Tuberculosis
J. Immunol.,
January 15, 2006;
176(2):
1131 - 1140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Ely, T. Cookenham, A. D. Roberts, and D. L. Woodland
Memory T Cell Populations in the Lung Airways Are Maintained by Continual Recruitment
J. Immunol.,
January 1, 2006;
176(1):
537 - 543.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. A. W. Claassen, P. A. A. van der Kant, Z. S. Rychnavska, G. M. van Bleek, A. J. Easton, and R. G. van der Most
Activation and Inactivation of Antiviral CD8 T Cell Responses during Murine Pneumovirus Infection
J. Immunol.,
November 15, 2005;
175(10):
6597 - 6604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. L. Woodland and I. Scott
T Cell Memory in the Lung Airways
Proceedings of the ATS,
August 1, 2005;
2(2):
126 - 131.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Cruise, H. M. Melief, J. Lukens, C. Soguero, and Y. S. Hahn
Increased Fas ligand expression of CD4+ T cells by HCV core induces T cell-dependent hepatic inflammation
J. Leukoc. Biol.,
August 1, 2005;
78(2):
412 - 425.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Kandula and C. Abraham
LFA-1 on CD4+ T Cells Is Required for Optimal Antigen-Dependent Activation In Vivo
J. Immunol.,
October 1, 2004;
173(7):
4443 - 4451.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. C. Whipple, R. S. Shanahan, A. H. Ditto, R. P. Taylor, and M. A. Lindorfer
Analyses of the In Vivo Trafficking of Stoichiometric Doses of an Anti-Complement Receptor 1/2 Monoclonal Antibody Infused Intravenously in Mice
J. Immunol.,
August 15, 2004;
173(4):
2297 - 2306.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. W. Lawrence and T. J. Braciale
Activation, Differentiation, and Migration of Naive Virus-Specific CD8+ T Cells during Pulmonary Influenza Virus Infection
J. Immunol.,
July 15, 2004;
173(2):
1209 - 1218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. John and I. N. Crispe
Passive and Active Mechanisms Trap Activated CD8+ T Cells in the Liver
J. Immunol.,
May 1, 2004;
172(9):
5222 - 5229.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. H. Ely, A. D. Roberts, and D. L. Woodland
Cutting Edge: Effector Memory CD8+ T Cells in the Lung Airways Retain the Potential to Mediate Recall Responses
J. Immunol.,
October 1, 2003;
171(7):
3338 - 3342.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
