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Prepublished online as a Blood First Edition Paper on January 9, 2003; DOI 10.1182/blood-2002-10-3175. This Article Full Text (PDF) All Versions of this Article: 2002-10-3175v1 101/10/4029    most recent Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Rights and Permissions Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Oppezzo, P. Articles by Dighiero, G. Search for Related Content PubMed PubMed Citation Articles by Oppezzo, P. Articles by Dighiero, G. Social Bookmarking                   What's this? Submitted October 21, 2002 Accepted December 23, 2002 Chronic lymphocytic leukemia B cells expressing AID display a dissociation between class switch recombination and somatic hypermutation Pablo Oppezzo, Francoise Vuillier, Yuri Vasconcelos, Gerard Dumas, Christian Magnac, Beatrice Payelle-Brogard, Otto Pritsch, and Guillaume Dighiero* Unite d'Immuno-hematologie, Institut Pasteur, Paris, France Department of Hematology, Universidad Federal de Sao Paulo, Sao Paulo, SP, Brazil Department of Biochemistry, Facultad de Medicina, Montevideo, Uruguay * Corresponding author; email: dighiero{at}pasteur.fr. In B-cells, somatic hypermutation (SHM) and class switch recombination (CSR) depend on the activation-induced deaminase (AID) gene product, although its precise mode of action remains unknown. Since some chronic lymphocytic leukemia (CLL) B-cells can undergo CSR without SHM, it constitutes a useful model to dissect AID function. In this work, we have studied AID expression, the presence of mutations in pre-switch µ DNA region, CSR and the SHM in 65 CLL patients. Our results demonstrate that: 1) unmutated CLL B-cells can constitutively express AID and its expression is associated to the presence of mutations in the pre-switch region and clonally related isotype-switched transcripts and 2) in CLL without constitutive AID expression, its induction upon stimulation results in pre-switch mutations and CSR process. Our results show a dissociation between SHM and CSR in CLL and suggest that, in this disease, AID would require additional help to carry out SHM process. CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this? This article has been cited by other articles: X. Wu, J. R. Darce, S. K. Chang, G. S. Nowakowski, and D. F. 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